New Ceratocystis species from Eucalyptus and Cunninghamia in South China

Show simple item record Liu, FeiFei Mbenoun, Michael Barnes, Irene Roux, Jolanda Wingfield, Michael J. Li, GuoQing Li, JieQiong Chen, ShuaiFei 2015-07-02T07:21:35Z 2015-07-02T07:21:35Z 2015-06
dc.description.abstract During routine surveys for possible fungal pathogens in the rapidly expanding plantations of Eucalyptus and Cunninghamia lanceolata in China, numerous isolates of unknown species in the genus Ceratocystis (Microascales) were obtained from tree wounds. In this study we identified the Ceratocystis isolates from Eucalyptus and Cunninghamia in the GuangDong, GuangXi, FuJian and HaiNan Provinces of South China based on morphology and through comparisons of DNA sequence data for the ITS, partial β-tubulin and TEF-1α gene regions. Morphological and DNA sequence comparisons revealed two previously unknown species residing in the Indo-Pacific Clade. These are described here as C. cercfabiensis sp. nov. and C. collisensis sp. nov. Isolates of C. cercfabiensis showed intragenomic variation in their ITS sequences and four strains were selected for cloning of the ITS gene region. Twelve ITS haplotypes were obtained from 17 clones selected for sequencing,differing in up to seven base positions and representing two separate phylogenetic groups. This is the first evidence of multiple ITS types in isolates of Ceratocystis residing in the Indo-Pacific Clade. Caution should thus be exercised when using the ITS gene region as a barcoding marker for Ceratocystis species in this clade. This study also represents the first record of a species of Ceratocystis from Cunninghamia. en_ZA
dc.description.embargo 2016-06-30 en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship This study was initiated through the bilateral agreement between the Governments South Africa and China, and we are grateful for the funding via projects 2012DFG31830 (International Science & Technology Cooperation Program of China), 31400546 (National Natural Science Foundation of China: NSFC),2010KJCX015-03 (Forestry Science and Technology Innovation Project of Guangdong Province of China). The Tree Protection and Cooperation Programme (TPCP) and the National ResearchFoundation (NRF), South Africa. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Liu, FF, Mbenoun, M, Barnes, I, Roux, J, Wingfield, MJ, Li, GQ, Li, JQ & Chen, SF 2015, ' New Ceratocystis species from Eucalyptus and Cunninghammia in South China', Antonie van Leeuwenhoek, vol. 107, no. 6, pp. 1451-1473. en_ZA
dc.identifier.issn 0003-6072 (print)
dc.identifier.issn 1572-9699 (online)
dc.identifier.other 10.1007/s10482-015-0441-3
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Springer International Publishing Switzerland 2015. The original publication is available at : http://link.springer.comjournal/10482 en_ZA
dc.subject Ceratocystidaceae en_ZA
dc.subject Fungal barcoding genes en_ZA
dc.subject Multiple ITS types en_ZA
dc.subject Plantation forestry en_ZA
dc.title New Ceratocystis species from Eucalyptus and Cunninghamia in South China en_ZA
dc.type Postprint Article en_ZA

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