Anti-inflammatory, anticholinesterase and antioxidant activity of leaf extracts of twelve plants used traditionally to alleviate pain and inflammation in South Africa

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dc.contributor.author Dzoyem, Jean Paul
dc.contributor.author Eloff, Jacobus Nicolaas
dc.date.accessioned 2015-07-01T05:15:56Z
dc.date.available 2015-07-01T05:15:56Z
dc.date.issued 2015-02
dc.description.abstract ETHNOPHARMAOLOGICAL RELEVANCE :Oxidative stress and inflammatory conditions are among the pathological features associated with the central nervous system in Alzheimer’s disease. Traditionally, medicinal plants have been used to alleviate inflammation, pains and also other symptoms possibly associated with Alzheimer’s disease. Therefore, the present study was designed to determine the in vitro antiinflammatory, antioxidant and anticholinesterase activity of twelve South African medicinal plants traditionally used to alleviate pain and inflammation. MATERIALS AND METHODS : Nitric oxide (NO) production in LPS-activated RAW 264.7 macrophages and 15-lipoxygenase (LOX) inhibitory assay were used to evaluate the anti-inflammatory activity. Acetylcholinesterase inhibition was assessed by using a modification of the Ellman’s method. Antioxidant activity, total phenolic and total flavonoids contents were determined using standard in vitro methods. RESULTS : The extract of Burkea africana had the highest anti-15-lipoxygenase activity with 85.92% inhibition at 100 μg/mL. All the extracts tested inhibited nitric oxide (NO) production in a dose dependant manner in LPS-stimulated RAW 264.7 macrophages. However, extracts from Leucaena leucocephala, Lippia javanica inhibited the production of NO by 97% at a concentration of 25 μg/mL. In addition, both Leucaena leucocephala and Englerophytum magaliesmontanum had strong activity against acetylcholinesterase with IC50 values of 118 μg/mL and 160 μg/mL respectively. Hight levels of phenolics and flavonoids were found in Leucaena leucocephala, Lippia javanica and Burkea africana. The correlation with antioxidant activities was not strong indicating that other metabolites may also be involved in antioxidant activity. CONCLUSIONS : The results obtained in this study validate the use of leaf extracts of these plants in South African traditional medicine against inflammation. Extracts of these plants species might be of value in the management of various diseases emerging from oxidative stress and related degenerative disorders. en_ZA
dc.description.embargo 2016-02-28 en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship National Research Foundation (NRF) and Medical Research Council (MRC). en_ZA
dc.description.uri http://www.elsevier.com/locate/jethpharm en_ZA
dc.identifier.citation Dzoyem, JP & Eloff, JN 2015, 'Anti-inflammatory, anticholinesterase and antioxidant activity of leaf extracts of twelve plants used traditionally to alleviate pain and inflammation in South Africa', Journal of Ethnopharmacology, vol. 160, no. 2, pp. 194-201. en_ZA
dc.identifier.issn 0378-8741 (print)
dc.identifier.issn 1872-7573 (online)
dc.identifier.other 10.1016/j.jep.2014.11.034
dc.identifier.uri http://hdl.handle.net/2263/45828
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2014 Elsevier Ireland Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Ethnopharmacology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Ethnopharmacology, vol. 160, no. 2, pp. 194-201. doi: 10.1016/j.jep.2014.11.034 en_ZA
dc.subject Medicinal plants en_ZA
dc.subject Anti-lipoxygenase en_ZA
dc.subject Nitric oxide production inhibition en_ZA
dc.subject Anticholinesterase en_ZA
dc.subject Antioxidants en_ZA
dc.title Anti-inflammatory, anticholinesterase and antioxidant activity of leaf extracts of twelve plants used traditionally to alleviate pain and inflammation in South Africa en_ZA
dc.type Postprint Article en_ZA


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