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dc.contributor.author | Dzoyem, Jean Paul | |
dc.contributor.author | Nkuete, Antoine H.L. | |
dc.contributor.author | Ngameni, Barthelemy | |
dc.contributor.author | Eloff, Jacobus Nicolaas | |
dc.date.accessioned | 2015-07-01T05:08:51Z | |
dc.date.available | 2015-07-01T05:08:51Z | |
dc.date.issued | 2017-10 | |
dc.description.abstract | This study was aimed at investigating the anti-inflammatory and anticholinesterase activity of six naturally occurring flavonoids: (-) pinostrobin (1), 2’,4’-dihydroxy-3’,6’-dimethoxychalcone (2), 6-8-diprenyleriodictyol (3), isobavachalcone (4), 4-hydroxylonchocarpin (5) and 6- prenylapigenin (6). These compounds were isolated from Dorstenia and Polygonum species used traditionally to treat pain. The anti-inflammatory activity was determined by using the Griess assay and the 15-lipoxygenase inhibitory activity was determined with the ferrous oxidationxylenol orange assay. Acetylcholinesterase (AChE) inhibition was determined by the Ellman’s method. At the lowest concentration tested (3.12 μg/mL), compounds 2, 3 and 4 had significant NO inhibitory activity with 90.71%, 84.65 and 79.57% inhibition respectively compared to the positive control quercetin (67.93%). At this concentration there was no significant cytotoxicity against macrophages with 91.67%, 72.86 and 70.86% cell viability respectively, compared to 73.1% for quercetin. Compound 4 had the most potent lipoxygenase inhibitory activity (IC50 of 25.92 μg/mL). With the exception of (-) pinostrobin (1), all the flavonoids had selective anticholinesterase activity with IC50 values ranging between 5.93 and 8.76 μg/mL compared to the IC50 4.94 μg/mL of eserine the positive control. These results indicate that the studied flavonoids especially isobavachalcone are potential anti-inflammatory natural products that may have the potential to be developed as therapeutic agents against inflammatory conditions and even Alzheimer’s disease. | en_ZA |
dc.description.embargo | 2016-05-08 | en_ZA |
dc.description.librarian | hb2015 | en_ZA |
dc.description.sponsorship | National Research Foundation (NRF) and Medical Research Council (MRC). | en_ZA |
dc.description.uri | http://link.springer.com/journal/12272 | en_ZA |
dc.identifier.citation | Dzoyem, J.P., Nkuete, A.H.L., Ngameni, B. et al. Anti-inflammatory and anticholinesterase activity of six flavonoids isolated from Polygonum and Dorstenia species. Archives of Pharmacal Research. (2017) 40: 1129-1134. https://doi.org/10.1007/s12272-015-0612-9. | en_ZA |
dc.identifier.issn | 0253-6269 (print) | |
dc.identifier.issn | 1976-3786 (online) | |
dc.identifier.other | 10.1007/s12272-015-0612-9 | |
dc.identifier.uri | http://hdl.handle.net/2263/45827 | |
dc.language.iso | en | en_ZA |
dc.publisher | Springer | en_ZA |
dc.rights | © The Pharmaceutical Society of Korea 2015. The original publication is available at : http://link.springer.com/journal/12272 | en_ZA |
dc.subject | Flavonoids | en_ZA |
dc.subject | 15-Lipoxygenase | en_ZA |
dc.subject | Nitric oxide production inhibition | en_ZA |
dc.subject | Anticholinesterase | en_ZA |
dc.title | Anti-inflammatory and anticholinesterase activity of six flavonoids isolated from Polygonum and Dorstenia species | en_ZA |
dc.type | Postprint Article | en_ZA |