dc.contributor.author |
Musengi, Amos
|
|
dc.contributor.author |
Khan, Nuraan
|
|
dc.contributor.author |
Le Roes-Hill, Marilize
|
|
dc.contributor.author |
Pletschke, Brett I.
|
|
dc.contributor.author |
Burton, Stephanie G.
|
|
dc.date.accessioned |
2015-06-30T07:23:23Z |
|
dc.date.available |
2015-06-30T07:23:23Z |
|
dc.date.issued |
2014-03 |
|
dc.description.abstract |
AIMS : To optimise peroxidase production by Streptomyces sp. strain BSII#1, up to 3 l culture
volumes.
METHODS AND RESULTS : Peroxidase production by Streptomyces sp. strain BSII#1 was
optimised in terms of production temperature and pH and the use of lignin-based model
chemical inducers. The highest peroxidase activity (1.30±0.04 U ml-1) in 10 ml culture
volume was achieved in a complex production medium (pH 8) at 37°C in the presence of 0.1
mmol l-1 veratryl alcohol, which was greater than those reported previously. Scale-up to 100 ml and 400 ml culture volumes resulted in decreased peroxidase production (0.53±0.10 U ml-
1 and 0.26±0.08 U ml-1 respectively). However, increased aeration improved peroxidase
production with the highest production achieved using an airlift bioreactor (4.76±0.46 U ml-1
in 3 l culture volume).
CONCLUSIONS : Veratryl alcohol (0.1 mmol l-1) is an effective inducer of peroxidase production
by Streptomyces sp. strain BSII#1. However, improved aeration increased peroxidase
production in larger volumes without the use of an inducer, surpassing induced yields in an
optimised small-scale process.
SIGNIFICANCE AND IMPACT OF THE STUDY : Only a limited number of reports in literature have
focused on the up-scaling of bacterial peroxidase production. There remains opportunity for
feasible large-scale production of bacterial peroxidases with potentially novel biocatalytic
properties. |
en_ZA |
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
National Research Foundation (NRF) of South Africa,Water Research Commission. |
en_ZA |
dc.description.uri |
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2672 |
en_ZA |
dc.identifier.citation |
Musengi, A, Khan, N, Le Roes-Hill, M, Pletschke, BI & Burton, SG 2014, 'Increasing the scale of peroxidase production by Streptomyces sp. strain BSII#1', Journal of Applied Microbiology, vol. 116, no. 3, pp. 554-562. |
en_ZA |
dc.identifier.issn |
1364-5072 (print) |
|
dc.identifier.issn |
1365-2672 (online) |
|
dc.identifier.other |
10.1111/jam.12380 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/45817 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Wiley |
en_ZA |
dc.rights |
© 2013 The Society for Applied Microbiology. This is the pre-peer reviewed version of the following article : Increasing the scale of peroxidase production by Streptomyces sp. strain BSII#1, Journal of Applied Microbiology, vol. 116, no. 3, pp. 554-562, 2014. doi : 10.1111/jam.12380. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1365-2672 |
en_ZA |
dc.subject |
Peroxidase |
en_ZA |
dc.subject |
Actinomycete |
en_ZA |
dc.subject |
Biocatalysis |
en_ZA |
dc.title |
Increasing the scale of peroxidase production by Streptomyces sp. strain BSII#1 |
en_ZA |
dc.type |
Postprint Article |
en_ZA |