Molecular detection of Mycobacterium tuberculosis from sputum transported in PrimeStore(®) from rural settings
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Date
Authors
Daum, Luke T.
Peters, Remco P.H.
Jonkman, K.
Worthy, Sue A.
Rodriguez, John D.
Ismail, Nazir Ahmed
Omar, Shaheed Vally
Fischer, Gerald W.
Journal Title
Journal ISSN
Volume Title
Publisher
International Union Against Tuberculosis and Lung Disease
Abstract
SETTING : Mopani District, South Africa.
OBJECTIVE : To explore remote, molecular detection of
Mycobacterium tuberculosis from sputum transported
using PrimeStorew Molecular Transport Medium (PSMTM)
compared to settings where microscopy or
Xpertw MTB/RIF is used as the baseline test.
DESIGN : Two sputum specimens were collected from
patients with cough of72 weeks at clinics in rural South
Africa. Shortly after expectoration and before processing
using Xpert, microscopy and liquid culture, a flocked
swab was swirled in each of these specimens and placed
in PS-MTM. Swabs were stored and transported to the
United States at ambient temperature for real-time
PrimeMixw polymerase chain reaction (PM-PCR).
RESULTS : Of 132 patients, 23 (17%) were positive on
microscopy, 39 (30%) on Xpert and 44 (33%) by PSMTM/PSMTM/
PM-PCR. Concordance of PS-MTM/PM-PCR
with positive microscopy and Xpert was respectively
96% and 85%. Of 107 microscopy-negative samples, 22
(21%) were positive using PS-MTM/PM-PCR, while
11/91 (12%) Xpert-negative samples were PS-MTM/
PM-PCR-positive. PS-MTM/PM-PCR positivity was
significantly higher than smear microscopy positivity
(P , 0.001), but similar to Xpert (P ¼ 0.33).
CONCLUSION: PCR testing of specimens transported in
PS-MTM would enhance TB diagnosis in settings where
smear microscopy is the baseline diagnostic test, and
could provide an alternative in settings where Xpert
testing is not available.
Description
Keywords
Specimen transport, PrimeMix, Real-time PCR, Specimen detection, Mycobacterium tuberculosis (MTB), PrimeStore(®) Molecular Transport Medium (PS-MTM)
Sustainable Development Goals
Citation
Daum, LT, Peters, RPH, Fourie, PB, Jonkman, K, Worthy, SA, Rodriguez, JD, Ismail, NA, Omar, SV & Fischer, GW 2015, 'Molecular detection of Mycobacterium tuberculosis from sputum transported in PrimeStore(®) from rural settings', International Journal of Tuberculosis and Lung Disease, vol.19, no. 5, pp. 552-557.