Evaluating the resolution power of new microsatellites for species identification and stock delimitation in the Cape hakes Merluccius paradoxus and Merluccius capensis (Teleostei : Merlucciidae)
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Date
Authors
Hoareau, Thierry B.
Klopper, Arrie Willem
Dos Santos, S.M.R.
Oosthuizen, Carel Jakobus
Bloomer, Paulette
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
The utility of 15 new and 17 previously published microsatellite markers was evaluated for
species identification and stock delimitation in the deep-water hake Merluccius paradoxus and
the shallow-water hake Merluccius capensis. A total of 14 microsatellites was polymorphic in M.
paradoxus and 10 in M. capensis. Two markers could individually discriminate the species using
Bayesian clustering methods and a statistical power analysis showed that the set of markers for
each species is likely to detect subtle genetic differentiation (FST < 0.006), which will be valuable
to delimit and characterise genetic stocks.
Description
Keywords
Bayesian methods, Cross-species amplification, Genetic markers, Genomic library, Power analysis
Sustainable Development Goals
Citation
Hoareau, TB, Klopper, AW, Dos Santos, SMR, Oosthuizen, CJ & Bloomer, P 2015, 'Evaluating the resolution power of new microsatellites for species identification and stock delimitation in the Cape hakes Merluccius paradoxus and Merluccius capensis (Teleostei : Merlucciidae)', Journal of Fish Biology, vol. 86, no. 5, pp. 1650-1657.