Evaluating the resolution power of new microsatellites for species identification and stock delimitation in the Cape hakes Merluccius paradoxus and Merluccius capensis (Teleostei : Merlucciidae)

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Authors

Hoareau, Thierry B.
Klopper, Arrie Willem
Dos Santos, S.M.R.
Oosthuizen, Carel Jakobus
Bloomer, Paulette

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Journal ISSN

Volume Title

Publisher

Wiley

Abstract

The utility of 15 new and 17 previously published microsatellite markers was evaluated for species identification and stock delimitation in the deep-water hake Merluccius paradoxus and the shallow-water hake Merluccius capensis. A total of 14 microsatellites was polymorphic in M. paradoxus and 10 in M. capensis. Two markers could individually discriminate the species using Bayesian clustering methods and a statistical power analysis showed that the set of markers for each species is likely to detect subtle genetic differentiation (FST < 0.006), which will be valuable to delimit and characterise genetic stocks.

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Keywords

Bayesian methods, Cross-species amplification, Genetic markers, Genomic library, Power analysis

Sustainable Development Goals

Citation

Hoareau, TB, Klopper, AW, Dos Santos, SMR, Oosthuizen, CJ & Bloomer, P 2015, 'Evaluating the resolution power of new microsatellites for species identification and stock delimitation in the Cape hakes Merluccius paradoxus and Merluccius capensis (Teleostei : Merlucciidae)', Journal of Fish Biology, vol. 86, no. 5, pp. 1650-1657.