Species diversity of non-tuberculous mycobacteria isolated from humans, livestock and wildlife in the Serengeti ecosystem, Tanzania

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dc.contributor.author Katale, Bugwesa Z.
dc.contributor.author Mbugi, Erasto V.
dc.contributor.author Botha, Louise
dc.contributor.author Keyyu, Julius D.
dc.contributor.author Kendall, Sharon
dc.contributor.author Dockrell, Hazel M.
dc.contributor.author Michel, Anita Luise
dc.contributor.author Kazwala, Rudovick R.
dc.contributor.author Rweyemamu, Mark M.
dc.contributor.author Van Helden, Paul David
dc.contributor.author Matee, Mecky I.
dc.date.accessioned 2015-06-03T08:04:09Z
dc.date.available 2015-06-03T08:04:09Z
dc.date.issued 2014-11-18
dc.description.abstract BACKGROUND : Non-tuberculous mycobacteria (NTM), which are ubiquitous micro-organisms occurring in humans, animals and the environment, sometimes receive public health and veterinary attention as opportunistic disease-causing agents. In Tanzania, there is limited information regarding the diversity of NTM species, particularly at the human-livestock-wildlife interface such as the Serengeti ecosystem, where potential for cross species infection or transmission may exist. METHODS : Mycobacterial DNA was extracted from cultured isolates obtained from sputum samples of 472 suspect TB patients and 606 tissues from wildlife species and indigenous cattle. Multiplex PCR was used to differentiate NTM from Mycobacterium tuberculosis complex (MTBC) members. NTM were further identified to species level by nucleotide sequencing of the 16S rRNA gene. RESULTS : A total of fifty five (55) NTM isolates representing 16 mycobacterial species and 5 isolates belonging to the MTBC were detected. Overall, Mycobacterium intracellulare which was isolated from human, cattle and wildlife, was the most frequently isolated species (20 isolates, 36.4%) followed by M. lentiflavum (11 isolates, 20%), M. fortuitum (4 isolates, 7.3%) and M. chelonae-abscessus group (3 isolates, 5.5%). In terms of hosts, 36 isolates were from cattle and 12 from humans, the balance being found in various wildlife species. CONCLUSION : This study reveals a diversity of NTM species in the Serengeti ecosystem, some of which have potential for causing disease in animals and humans. The isolation of NTM from tuberculosis-like lesions in the absence of MTBC calls for further research to elucidate their actual role in causing disease. We are also suggesting a one health approach in identifying risk factors for and possible transmission mechanisms of the NTM in the agro-pastoral communities in the Serengeti ecosystem. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.sponsorship The Welcome Trust Grant [WT087546MA] to the Southern African Centre for Infectious Diseases and Surveillance (SACIDS) and The Muhimbili University of Health and Allied Sciences (MUHAS) through its SIDA-Sarec small grant [000/3177]. en_ZA
dc.description.uri http://www.biomedcentral.com/bmcinfectdis/ en_ZA
dc.identifier.citation Katale, BZ, Mbugi, EV, Botha, L, Keyyu, JD, Kendall, S, Dockrell, HM, Michel, AL, Kazwala, RR, Rweyemamu, MM, Van Helden, PD & Matee, MI 2014, 'Species diversity of non-tuberculous mycobacteria isolated from humans, livestock and wildlife in the Serengeti ecosystem, Tanzania', BMC Infectious Diseases, vol. 14, no. art. no. 616, pp. 1-8. en_ZA
dc.identifier.issn 1471-2334
dc.identifier.other 10.1186/s12879-014-0616-y
dc.identifier.other N-8996-2014
dc.identifier.other 55978917900
dc.identifier.uri http://hdl.handle.net/2263/45379
dc.language.iso en en_ZA
dc.publisher BioMed Central en_ZA
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2014 Katale et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. en_ZA
dc.subject Species diversity en_ZA
dc.subject Human-animal interface en_ZA
dc.subject Serengeti ecosystem en_ZA
dc.subject Non-tuberculous mycobacteria (NTM) en_ZA
dc.title Species diversity of non-tuberculous mycobacteria isolated from humans, livestock and wildlife in the Serengeti ecosystem, Tanzania en_ZA
dc.type Article en_ZA


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