Ectopic phytocystatin expression increases nodule numbers and influences the responses of soybean (Glycine max) to nitrogen deficiency

Show simple item record Quain, Marian D. Makgopa, Matome Eugene Schnaubelt, Daniel Kunert, Karl J. Foyer, Christine H. Márquez-García, Belén Comadira, Gloria Fernandez-Garcia, Nieves Olmos, Enrique 2015-06-03T07:09:38Z 2015-06-03T07:09:38Z 2015-04
dc.description.abstract Cysteine proteases and cystatins havemany functions that remain poorly characterised, particularly in crop plants. We therefore investigated the responses of these proteins to nitrogen deficiency in wild-type soybeans and in two independent transgenic soybean lines (OCI-1 and OCI-2) that express the rice cystatin, oryzacystatin-I (OCI). Plants were grown for four weeks under either a high (5 mM) nitrate (HN) regime or in the absence of added nitrate (LN) in the absence or presence of symbiotic rhizobial bacteria. Under the LN regime all lines showed similar classic symptoms of nitrogen deficiency including lower shoot biomass and leaf chlorophyll. However, the LN-induced decreases in leaf protein and increases in root protein tended to be smaller in the OCI-1 and OCI-2 lines than in the wild type. When LN-plants were grown with rhizobia, OCI-1 and OCI-2 roots had significantly more crown nodules than wild-type plants. The growth nitrogen regime had a significant effect on the abundance of transcripts encoding vacuolar processing enzymes (VPEs), LN-dependent increases in VPE2 and VPE3 transcripts in all lines. However, the LN-dependent increases of VPE2 and VPE3 transcripts were significantly lower in the leaves of OCI-1 and OCI-2 plants than in the wild type. These results show that nitrogen availability regulates the leaf and root cysteine protease, VPE and cystatin transcript profiles in a manner that is in some cases influenced by ectopic OCI expression. Moreover, the OCI-dependent inhibition of papain-like cysteine proteases favours increased nodulation and enhanced tolerance to nitrogen limitation, as shown by the smaller LN-dependent decreases in leaf protein observed in the OCI-1 and OCI-2 plants relative to the wild type. en_ZA
dc.description.embargo 2016-04-30 en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship FP7-PIRSES-GA-2008-230830 (LEGIM) and PIIF-GA-2011-299347 (Soylife; K.K.). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Quain, MD, Makgopa, ME, Cooper, JW, Kunert, KJ & Foyer, CH 2015, 'Ectopic phytocystatin expression increases nodule numbers and influences the responses of soybean (Glycine max) to nitrogen deficiency', Phytochemistry, vol. 112, pp. 179-187. en_ZA
dc.identifier.issn 0031-9422 (print)
dc.identifier.issn 1873-3700 (online)
dc.identifier.other 10.1016/j.phytochem.2014.12.027
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2015 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Phytochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Phytochemistry, vol.112, pp. 179-187, 2015. doi :10.1016/j.phytochem.2014.12.027 en_ZA
dc.subject Cystatin en_ZA
dc.subject Cysteine protease en_ZA
dc.subject Lateral root density en_ZA
dc.subject Leaf senescence en_ZA
dc.subject Nitrogen deficiency en_ZA
dc.subject Nodulation en_ZA
dc.subject Vacuolar processing enzymes en_ZA
dc.title Ectopic phytocystatin expression increases nodule numbers and influences the responses of soybean (Glycine max) to nitrogen deficiency en_ZA
dc.type Postprint Article en_ZA

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