Resolving the polyphyletic nature of Pyricularia (Pyriculariaceae)

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dc.contributor.author Kaublauf, Sylvia
dc.contributor.author Tharreau, D.
dc.contributor.author Fournier, E.
dc.contributor.author Groenewald, Johannes Z.
dc.contributor.author Crous, Pedro W.
dc.contributor.author De Vries, Ronald P.
dc.contributor.author Lebrun, M.-H.
dc.date.accessioned 2015-05-28T11:22:15Z
dc.date.available 2015-05-28T11:22:15Z
dc.date.issued 2014-10-25
dc.description.abstract Species of Pyricularia (magnaporthe-like sexual morphs) are responsible for major diseases on grasses. Pyricularia oryzae (sexual morph Magnaporthe oryzae) is responsible for the major disease of rice called rice blast disease, and foliar diseases of wheat and millet, while Pyricularia grisea (sexual morph Magnaporthe grisea) is responsible for foliar diseases of Digitaria. Magnaporthe salvinii, M. poae and M. rhizophila produce asexual spores that differ from those of Pyricularia sensu stricto that has pyriform, 2-septate conidia produced on conidiophores with sympodial proliferation. Magnaporthe salvinii was recently allocated to Nakataea, while M. poae and M. rhizophila were placed in Magnaporthiopsis. To clarify the taxonomic relationships among species that are magnaporthe- or pyricularia-like in morphology, we analysed phylogenetic relationships among isolates representing a wide range of host plants by using partial DNA sequences of multiple genes such as LSU, ITS, RPB1, actin and calmodulin. Species of Pyricularia s. str. belong to a monophyletic clade that includes all P. oryzae/P. grisea isolates tested, defining the Pyriculariaceae, which is sister to the Ophioceraceae, representing two novel families. These clades are clearly distinct from species belonging to the Gaeumannomyces pro parte/Magnaporthiopsis/Nakataea generic complex that are monophyletic and define the Magnaporthaceae. A few magnaporthe- and pyricularia-like species are unrelated to Magnaporthaceae and Pyriculariaceae. Pyricularia oryzae/P. grisea isolates cluster into two related clades. Host plants such as Eleusine, Oryza, Setaria or Triticum were exclusively infected by isolates from P. oryzae, while some host plant such as Cenchrus, Echinochloa, Lolium, Pennisetum or Zingiber were infected by different Pyricularia species. This demonstrates that host range cannot be used as taxonomic criterion without extensive pathotyping. Our results also show that the typical pyriform, 2-septate conidium morphology of P. grisea/P. oryzae is restricted to Pyricularia and Neopyricularia, while most other genera have obclavate to more ellipsoid 2-septate conidia. Some related genera (Deightoniella, Macgarvieomyces) have evolved 1-septate conidia. Therefore, conidium morphology cannot be used as taxonomic criterion at generic level without phylogenetic data. We also identified 10 novel genera, and seven novel species. A re-evaluation of generic and species concepts within Pyriculariaceae is presented, and novelties are proposed based on morphological and phylogenetic data. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.uri http://www.studiesinmycology.org/ en_ZA
dc.identifier.citation Klaubauf, S, Tharreau, D Fournier, E, Groenewald, JZ, Crous, PW, De Vries, RP & Lebrun, M-H 2014, 'Resolving the polyphyletic nature of Pyricularia (Pyriculariaceae)', Studies in Mycology, vol. 79, pp. 85-120. en_ZA
dc.identifier.issn 0166-0616 (print)
dc.identifier.issn 1872-9797 (online)
dc.identifier.other 10.1016/j.simyco.2014.09.004
dc.identifier.uri http://hdl.handle.net/2263/45329
dc.language.iso en en_ZA
dc.publisher Fungal Biodiversity Centre (CBS) en_ZA
dc.rights © 2014, CBS-KNAW Fungal Biodiversity Centre. en_ZA
dc.subject Magnaporthaceae en_ZA
dc.subject Magnaporthe en_ZA
dc.subject Pyricularia en_ZA
dc.subject Pyriculariaceae en_ZA
dc.subject Phylogeny en_ZA
dc.subject Systematics en_ZA
dc.title Resolving the polyphyletic nature of Pyricularia (Pyriculariaceae) en_ZA
dc.type Article en_ZA


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