Molecular characterization of Theileria parva field strains derived from cattle and buffalo sympatric populations of Northern Tanzania

Show simple item record Elisa, Mwega Gwakisa, Paul Sibeko-Matjila, Kgomotso Penelope Oosthuizen, Marinda C. Geysen, Dirk 2015-05-26T06:14:55Z 2015-05-26T06:14:55Z 2014
dc.description.abstract East coast fever is a disease of cattle caused by Theileria parva and is transmitted by the tick Rhipicephalus appendiculatus. Buffalos are a natural reservoir of the parasite. The objectives of this study were to determine the T. parva infection in buffalo and cattle field samples, to identify genotypes of T. parva infecting cattle and buffalo and finally to investigate the presence of buffalo-derived T. parva genotypes in cattle that graze in close proximity with infected buffalo. Real-Time PCR (RT-PCR) and Fluorescence Resonance Energy Transfer (FRET) was used to diagnose T.parva. Genotyping of T. parva was conducted by analysing p67, PIM and p104 genes . In addition, amplicons with unique PIM and p104 PCR-RFLP profiles were sequenced to identify shared genotypes. The results have shown the high occurrence of T. parva in buffalo and a much higher occurrence of T. parva in cattle from the Ngorongoro area. Analysis of the variable region of the p67 loci showed four different T. parva alleles in buffalo and cattle. PCR-RFLP analysis of the p104 loci revealed four allelic profiles in buffalo and two in cattle. PCR-RFLP analysis of PIM loci from buffalo gave profiles that were complex and difficult to interpret. On the contrary, cattle showed allelic profiles that resemble Muguga and Marikebuni isolate PIM gene profiles. Multi-locus genotypes (MLGs) and sequence analysis revealed that the majority of buffalo and cattle genotypes were distinct but there is an indication of some sharing of genotypes between buffalo and cattle. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.sponsorship Belgian Directorate General for Development Co-operation Framework agreement ITM/DGCD and the Institute of Tropical Medicine (ITM). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Mwega Elisa, Paul Gwakisa, Kgomotso Sibeko, Marinda Oosthuizen, Dirk Geysen. Molecular characterization of Theileria parva field strains derived from Cattle and Buffalo sympatric populations of Northern Tanzania. American Journal of Research Communication, 2014, 2(7): 10-22. en_ZA
dc.identifier.issn 2325-4076
dc.identifier.other 24332907600
dc.identifier.other O-6028-2014
dc.identifier.other N-8706-2014
dc.identifier.other 7004592997
dc.language.iso en en_ZA
dc.publisher American Journal of Research Communication en_ZA
dc.relation.requires Adobe Acrobat Reader en
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. en_ZA
dc.subject Theileria parva en_ZA
dc.subject East Coast fever en_ZA
dc.subject Buffalo en_ZA
dc.subject Cattle -- Diseases en_ZA
dc.subject Real-time PCR en_ZA
dc.subject Fluorescence resonance energy transfer (FRET) en_ZA
dc.subject p104 en_ZA
dc.subject p67 en_ZA
dc.subject Polymorphic immunodominant (PIM) en_ZA
dc.subject Amplicon en_ZA
dc.subject Allele en_ZA
dc.subject Northern Tanzania en_ZA
dc.subject RT-PCR
dc.title Molecular characterization of Theileria parva field strains derived from cattle and buffalo sympatric populations of Northern Tanzania en_ZA
dc.type Article en_ZA

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