Lumpy skin disease : attempted propagation in tick cell lines and presence of viral DNA in field ticks collected from naturally-infected cattle

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dc.contributor.author Tuppurainen, Eeva S.M.
dc.contributor.author Venter, Estelle Hildegard
dc.contributor.author Coetzer, Jacobus A.W.
dc.contributor.author Bell-Sakyi, Lesley
dc.date.accessioned 2015-05-25T09:13:11Z
dc.date.available 2015-05-25T09:13:11Z
dc.date.issued 2015-03
dc.description.abstract Lumpy skin disease (LSD) is of substantial economic importance for the cattle industry in Africa and the Near and Middle East. Several insect species are thought to transmit the disease mechanically. Recent transmission studies have demonstrated the first evidence for a role of hard (ixodid) ticks as vectors of lumpy skin disease virus (LSDV). The aim of this study was to attempt in vitro growth of the virus in Rhipicephalus spp. tick cell lines and investigate in vivo the presence of the virus in ticks collected from cattle during LSD outbreaks in Egypt and South Africa. No evidence was obtained for replication of LSDV in tick cell lines although the virus was remarkably stable, remaining viable for 35 days at 28 ◦C in tick cell cultures, in growth medium used for tick cells and in phosphate buffered saline. Viral DNA was detected in two-thirds of the 56 field ticks, making this the first report of the presence of potentially virulent LSDV in ticks collected from naturally infected animals. en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship Department for Environment, Food and Rural Affairs (DEFRA) and BBSRC. en_ZA
dc.description.uri http://www.elsevier.com/locate/ttbdis en_ZA
dc.identifier.citation Tuppurainen, ESM, Venter, EH, Coetzer, JAW & Bell-Sakyi, L 2015, 'Lumpy skin disease : attempted propagation in tick cell lines and presence of viral DNA in field ticks collected from naturally-infected cattle', Ticks and Tick-Borne Diseases, vol. 6, no. 2, pp. 134-140. en_ZA
dc.identifier.issn 1877-959X (print)
dc.identifier.issn 1877-9603 (online)
dc.identifier.other 10.1016/j.ttbdis.2014.11.002
dc.identifier.other O-6953-2014
dc.identifier.other 7003904650
dc.identifier.uri http://hdl.handle.net/2263/45251
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.relation.requires Adobe Acrobat Reader en
dc.rights Crown Copyright © 2014 Published by Elsevier GmbH. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). en_ZA
dc.subject Ticks en_ZA
dc.subject Tick cell lines en_ZA
dc.subject Rhipicephalus en_ZA
dc.subject Lumpy skin disease (LSD) en_ZA
dc.subject Lumpy skin disease virus (LSDV) en_ZA
dc.title Lumpy skin disease : attempted propagation in tick cell lines and presence of viral DNA in field ticks collected from naturally-infected cattle en_ZA
dc.type Article en_ZA


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