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dc.contributor.author | Abolnik, Celia![]() |
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dc.contributor.author | Gouws, Johan![]() |
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dc.date.accessioned | 2015-05-14T07:12:57Z | |
dc.date.available | 2015-05-14T07:12:57Z | |
dc.date.issued | 2014 | |
dc.description.abstract | The survival times of Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae (Ms) on washed and unwashed natural and synthetic kanekalon hair samples over a 5-d period were evaluated using the color changing unit method for comparison with results of previous studies conducted on natural hair. Regardless of whether synthetic or natural hair samples prewashed with a disinfectant shampoo were spiked with Mg or Ms, all viable organisms rapidly dropped below a count of 1 × 101/mL of culture. unwashed natural hair seeded with a titer of approximately 1 × 106/mL of viable Mg or Ms decreased to 6 × 105/mL and 6 × 103/ mL, respectively, by 4 h postseeding, but no viable Mg or Ms were detected on natural hair from 8 h onwards. By contrast, the titers of Mg and Ms on synthetic hair did not decline from the initial 1 × 106/mL seed dose up to 96 h postseeding, and, in fact, viable Mg and Ms was still detectable at 9 d postinfection. Application of a real-time quantitative single-tube duplex PCR assay confirmed that no proliferation of Mg or Ms had occurred on the synthetic hair samples, the cells simply remained viable. The unexpected finding that Mg and Ms survive for extended periods on synthetic kanekalon hair fibers raises the question of whether attachment to a surface is a prerequisite for the survival and persistence of Mg and Ms in the extra-host environment. Future studies should be aimed at determining whether other synthetic hair types or indeed other types of plastics commonly found in the poultry house offer similar survival advantages to mycoplasmas. | en_ZA |
dc.description.librarian | am2015 | en_ZA |
dc.description.sponsorship | The Southern African Poultry Association (Johannesburg). | en_ZA |
dc.description.uri | http://ps.oxfordjournals.org | en_ZA |
dc.identifier.citation | Abolnik, C & Gouws, J 2014, 'Extended survival times of Mycoplasma gallisepticum and Mycoplasma synoviae on kanekalon synthetic hair fibres', Poultry Science, vol. 93, pp. 8-11. | en_ZA |
dc.identifier.issn | 0032-5791 (print) | |
dc.identifier.issn | 1525-3171 (online) | |
dc.identifier.other | 10.3382/ps.2013-03457 | |
dc.identifier.other | N-9324-2014 | |
dc.identifier.other | 23093208700 | |
dc.identifier.uri | http://hdl.handle.net/2263/45156 | |
dc.language.iso | en | en_ZA |
dc.publisher | Oxford University Press | en_ZA |
dc.relation.requires | Adobe Acrobat Reader | en |
dc.rights | © 2014 Poultry Science Association Inc. | en_ZA |
dc.subject | Mycoplasma | en_ZA |
dc.subject | Synthetic | en_ZA |
dc.subject | Hair | en_ZA |
dc.subject | Survival | en_ZA |
dc.subject | PCR | en_ZA |
dc.subject | Mycoplasma gallisepticum (Mg) | en_ZA |
dc.subject | Mycoplasma synoviae (Ms) | en_ZA |
dc.title | Extended survival times of Mycoplasma gallisepticum and Mycoplasma synoviae on kanekalon synthetic hair fibres | en_ZA |
dc.type | Postprint Article | en_ZA |