Anti-bacterial and anti-oxidant activities of leaf extracts of Combretum vendae (Combretecacea) and the isolation of an anti-bacterial compound

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dc.contributor.author Komape, Nancy Patience Motlalepula
dc.contributor.author Aderogba, M.A. (Mutalib)
dc.contributor.author Bagla, Victor Patrick
dc.contributor.author Masoko, Peter
dc.contributor.author Eloff, Jacobus Nicolaas
dc.date.accessioned 2015-05-08T10:38:29Z
dc.date.available 2015-05-08T10:38:29Z
dc.date.issued 2014
dc.description.abstract BACKGROUND : Combretum vendae A.E. van Wyk (Combretaceae) is used for the treatment of bacterial related infections and oxidative related diseases by indigenous people of South Africa. Dried leaves extracts of C. vendae were investigated for bioactivity against a variety of bacterial strains and their antioxidant potential evaluated. MATERIALS AND METHODS : Constituents of leaf material were serially extracted using solvents of varying polarities, TLC chromatograms of the fractions were sprayed with 2,2 diphenyl-1-picrylhydrazyl (DPPH) to determine the presence of antioxidant compounds. Bio-autography was used to determine the number of antibacterial compounds active against Staphylococcus aureus, Enterococcus faecalis, Eschericha coli and Pseudomonas aeruginosa. Minimum inhibitory concentration (MIC) values were determined using serial microplate dilution method. The chloroform fraction was subjected to bio-assay guided column chromatography to isolate the active compound. RESULTS: The mass extracted by different solvents was below 10% dry weight. MIC values for different extracts against different pathogens ranges from 0.08 to 0.64 mg/ml. The compound isolated was identified as acacetin having an Rf value of 0.28 following elution in the Ethanol: Methanol: Water [E: M: W (10: 1.35: 1 v/v). Acacetin had MIC values ranging from 0.16 to 0.35 mg/ml. CONCLUSION : We report for the first time the isolation of acacetin as the main antibacterial compound from the leaves of Combretum vendae. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.sponsorship The National Research Foundation (NRF) en_ZA
dc.description.uri http://journals.sfu.ca/africanem/index.php/ajtcam en_ZA
dc.identifier.citation Komape, NPM, Aderogba, M, Bagla, VP, Masoko, P & Eloff, JN 2014, 'Anti-bacterial and anti-oxidant activities of leaf extracts of Combretum vendae (Combretecacea) and the isolation of an anti-bacterial compound', African Journal of Traditional, Complementary and Alternative Medicines (AJTCAM), vol. 11, no. 5, pp. 73-77. en_ZA
dc.identifier.issn 0189-814X
dc.identifier.uri http://hdl.handle.net/2263/45069
dc.language.iso en en_ZA
dc.publisher African Networks on Ethnomedicines en_ZA
dc.rights This work is licensed under the Creative Commons Attribution 3.0 Unported License. en_ZA
dc.subject Combretum vendae en_ZA
dc.subject Minimum inhibitory concentration en_ZA
dc.subject Bio-autography en_ZA
dc.subject Acacetin en_ZA
dc.subject Phytochemical analysis en_ZA
dc.subject Anti-oxidant en_ZA
dc.title Anti-bacterial and anti-oxidant activities of leaf extracts of Combretum vendae (Combretecacea) and the isolation of an anti-bacterial compound en_ZA
dc.type Article en_ZA


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