Addition of rabbit serum to EMJH medium improves isolation of Leptospira interrogans serovar hardjo

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Authors

Te Brugge, Lesley A.
Louw, H.N.

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Published by the Government Printer, Pretoria.

Abstract

The addition of 2 % pooled rabbit serum to semi-solid commercial EMJH medium with EMJH enrichment and 0,5 mg of 5-fluorouracil per ml was found to enhance the growth rate and success of isolation of Leptospira interrogans serovar hardjo from bovine urine. Cultures made on media without serum had to be kept for more than 130 days before being discarded as negative.

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The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.

Keywords

Veterinary medicine

Sustainable Development Goals

Citation

Te Brugge, LA & Louw, HN 1985, 'Addition of rabbit serum to EMJH medium improves isolation of Leptospira interrogans serovar hardjo’, Onderstepoort Journal of Veterinary Research, vol. 52, no. 1, pp. 53-54.