Molecular characterisation of hepatitis : a virus strains from water sources in South Africa

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dc.contributor.author Said, R.
dc.contributor.author Wolfaardt, Marianne
dc.contributor.author Taylor, Maureen B.
dc.date.accessioned 2015-03-26T13:01:32Z
dc.date.available 2015-03-26T13:01:32Z
dc.date.issued 2014-03
dc.description.abstract Hepatitis A virus (HAV) strains found in selected South African (SA) surface waters were characterised to establish what HAV types are circulating in the environment, thus reflecting circulation in the surrounding communities. Surface water samples used for irrigation or domestic purposes, and water samples from the outflow of wastewater plants were collected from six provinces. Viruses were recovered from the samples using a glass wool adsorption-elution method and then further concentrated using polyethylene glycol/sodium chloride precipitation. After automated nucleic acid extraction, samples were analysed for HAV by real-time reverse-transcriptase polymerase chain reaction. HAV strains were genotyped by nucleotide sequence analysis of the capsid gene VP1 and the VP1/P2B junction. HAVs were detected in 76% (16/21) of the surface water samples and in 37% (19/51) of the samples from the wastewater plants. Strains were characterised from 32 of the 35 samples and classified within genotype IB. The presence of genotype IB in the water sources confirms human faecal contamination. Hence, these faecally-contaminated water sources may be a potential transmission route of HAV infection and a potential source of contamination of irrigated fresh produce in SA. en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship Rachida Saïd was supported by a Poliomyelitis Research Foundation post-graduate bursary. This study was funded, in part, by the National Research Foundation (NRF), and in part by an on-going solicited research project (K5/ 1875//4) funded by the WRC and co-funded by the Department of Agriculture, Forestry and Fisheries, South Africa. en_ZA
dc.description.uri http://www.iwaponline.com/wst/toc.htm en_ZA
dc.identifier.citation Saïd, R, Wolfaardt, M & Taylor, MB 2014, 'Molecular characterisation of hepatitis : a virus strains from water sources in South Africa', Water Science and Technology, vol. 69, no. 5, pp. 923-933. en_ZA
dc.identifier.issn 0273-1223 (print)
dc.identifier.issn 1996-9732 (online)
dc.identifier.other 10.2166/wst.2013.799
dc.identifier.uri http://hdl.handle.net/2263/44187
dc.language.iso en en_ZA
dc.publisher IWA Publishing en_ZA
dc.rights © IWA Publishing 2014. The definitive peer-reviewed and edited version of this article is published in Water Science and Technology, vol. 69, no. 5, pp. 923-933, 2014. This journal is available at : http://www.iwaponline.com/wst/toc.htm en_ZA
dc.subject Genotypes en_ZA
dc.subject Irrigation water en_ZA
dc.subject Hepatitis A virus (HAV) en_ZA
dc.subject South Africa (SA) en_ZA
dc.title Molecular characterisation of hepatitis : a virus strains from water sources in South Africa en_ZA
dc.type Postprint Article en_ZA


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