Normalization of environmental metagenomic DNA enhances the discovery of under-represented microbial community members

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dc.contributor.author Ramond, Jean-Baptiste
dc.contributor.author Makhalanyane, Thulani P.
dc.contributor.author Tuffin, Marla I.
dc.contributor.author Cowan, Don A.
dc.date.accessioned 2015-03-26T08:15:54Z
dc.date.available 2015-03-26T08:15:54Z
dc.date.issued 2015-04
dc.description.abstract Normalization is a procedure classically employed to detect rare sequences in cellular expression profiles (i.e. cDNA libraries). Here, we present a normalization protocol involving the direct treatment of extracted environmental metagenomic DNA with S1 nuclease; referred to as Normalization of metagenomic DNA: NmDNA. We demonstrate that NmDNA, prior to post hoc PCR based experiments (16S rRNA gene T-RFLP fingerprinting and clone library), increased the diversity of sequences retrieved from environmental microbial communities by detection of rarer sequences. This approach could be used to enhance the resolution of detection of ecologically relevant rare members in environmental microbial assemblages. en_ZA
dc.description.embargo 2016-04-30 en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship National Research Foundation (NRF) of South Africa (BlueSkies program 81693). en_ZA
dc.description.uri http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1472-765X en_ZA
dc.identifier.citation Ramond, J.-B, Makhalanyane, TP, Tuffin, MI & Cowan, DA 2015, 'Normalization of environmental metagenomic DNA enhances the discovery of under-represented microbial community members', Letters in Applied Microbiology, vol. 60, no. 4, pp. 359-366. en_ZA
dc.identifier.issn 0266-8254 (print)
dc.identifier.issn 1472-765X (online)
dc.identifier.other 10.1111/lam.12380
dc.identifier.uri http://hdl.handle.net/2263/44172
dc.language.iso en en_ZA
dc.publisher Wiley en_ZA
dc.rights © 2014 The Society for Applied Microbiology. This is the pre-peer reviewed version of the following article : Normalization of environmental metagenomic DNA enhances the discovery of under-represented microbial community members, Letters in Applied Microbiology, vol. 60, no. 4, pp. 359-366, 2015, doi : 10.1111/lam.12380. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1472-765X. en_ZA
dc.subject Normalization en_ZA
dc.subject Environmental metagenomic en_ZA
dc.subject DNA enhances en_ZA
dc.subject Discovery en_ZA
dc.subject Novel microbial diversity en_ZA
dc.title Normalization of environmental metagenomic DNA enhances the discovery of under-represented microbial community members en_ZA
dc.type Postprint Article en_ZA


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