A high performance liquid chromatographic method for the fluorimetric determination of lactose, galactose and glucose in normal and abnormal milk of cows

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dc.contributor.author Kowalski, Z.E.
dc.contributor.author Giesecke, W.H.
dc.contributor.editor Bigalke, R.D.
dc.date.accessioned 2015-03-26T07:38:37Z
dc.date.available 2015-03-26T07:38:37Z
dc.date.created 2014
dc.date.issued 1986
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en_ZA
dc.description.abstract A high performance, liquid chromatographic, gradient method with post-column derivatization and highly sensitive fluorescence detection has been developed for the determination of reducing sugars, such as lactose, galactose and glucose, in normal and abnormal milk of cows. The method requires little sample preparation, is applicable to a fully automated HPLC system and has been found suitable for the processing of hundreds of samples. Peaks of minor constituent components of samples showed retention times identical with those of ribose, cellobiose, fucose, mannose and xylose. Further research with the HPLC technique, supplemented with modem gas chromatography and mass spectrometry, is considered necessary for elucidating the role of minor carbohydrates in the physiology and physiopathology of the bovine udder. en_ZA
dc.identifier.citation Kowalski, ZE & Giesecke, WH 1986, 'A high performance liquid chromatographic method for the fluorimetric determination of lactose, galactose and glucose in normal and abnormal milk of cows', Onderstepoort Journal of Veterinary Research, vol. 53, no. 4 , pp. 225-229. en_ZA
dc.identifier.issn 0330-2465
dc.identifier.uri http://hdl.handle.net/2263/44159
dc.language.iso en en_ZA
dc.publisher Published by the Government Printer, Pretoria. en_ZA
dc.rights ©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital). en_ZA
dc.subject Veterinary medicine en_ZA
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title A high performance liquid chromatographic method for the fluorimetric determination of lactose, galactose and glucose in normal and abnormal milk of cows en_ZA
dc.type Article en_ZA


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