Gene expression associated with intersterility in Heterobasidion

Show simple item record Van der Nest, Magrieta Aletta Olsen, Andreas Karlsson, M. Lind, Monica Dalman., K. Brandström-Durling, M. Elfstrand, M. Wingfield, Brenda D. Stenlid, J. 2015-03-18T09:52:09Z 2015-03-18T09:52:09Z 2014-12
dc.description.abstract Intersterility (IS) is thought to prevent mating compatibility between homokaryons that belong to different species. Although IS in Heterobasidion is regulated by the genes located at the IS loci, it is not yet known how the IS genes influence sexual compatibility and heterokaryon formation. To increase our understanding of the molecular events underlying IS, we studied mRNA abundance changes during IS compatible and incompatible interactions over time. The clustering of the transcripts into expression profiles, followed by the application of Gene Ontology (GO) enrichment pathway analysis of each of the clusters, allowed inference of biological processes participating in IS. These analyses identified events involved in mating and sexual development (i.e., linked with IS compatibility), which included processes associated with cell–cell adhesion and recognition, cell cycle control and signal transduction. We also identified events potentially involved in overriding mating between individuals belonging to different species (i.e., linked with IS incompatibility), which included reactive oxygen species (ROS) production, responses to stress (especially to oxidative stress), signal transduction and metabolic biosynthesis. Our findings thus enabled detection and characterization of gene expression changes associated with IS in Heterobasidion, as well as identification of important processes and pathways associated with this phenomenon. Overall, the results of this study increase current knowledge regarding the molecular mechanisms underpinning IS in Heterobasidion and allowed for the establishment of a vital baseline for further studies. en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship National Research Foundation, the Swedish Foundation for Strategic Research The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Uppsala Microbiomics Center). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Van der Nest, MA, Olson, A, Karlsson, M, Lind, M, Dalman, K, Brandstrom-Durling, M, Elfstrand, M, Wingfield, BD & Stenlid, J 2014, 'Gene expression associated with intersterility in Heterobasidion', Fungal Genetics and Biology, vol. 73, pp. 104-119. en_ZA
dc.identifier.issn 1087-1845 (print)
dc.identifier.issn 1096-0937 (online)
dc.identifier.other 10.1016/j.fgb.2014.10.008
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2014 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Fungal Genetics and Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Genetics and Biology, vol. 73, pp. 104-119, 2014. doi : 10.1016/j.fgb.2014.10.008 en_ZA
dc.subject Heterobasidion occidentale en_ZA
dc.subject Heterobasidion irregulare en_ZA
dc.subject Mating en_ZA
dc.subject RNA-seq en_ZA
dc.subject Intersterility (IS) en_ZA
dc.title Gene expression associated with intersterility in Heterobasidion en_ZA
dc.type Postprint Article en_ZA

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