Novel estradiol analogue induces apoptosis and autophagy in esophageal carcinoma cells

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Authors

Wolmarans, Elize
Mqoco, T.V. (Thandi Vuyelwa)
Stander, Andre
Nkandeu, Sandra
Sippel, Katherine H.
McKenna, Robert
Joubert, Annie M.

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Publisher

Springer

Abstract

Cancer is the second leading cause of death in South Africa. The critical role that microtubules play in cell division makes them an ideal target for the development of chemotherapeutic drugs that prevent the hyperproliferation of cancer cells. The new in silico-designed estradiol analogue 2-ethyl-3-O-sulfamoyl-estra-1,3,5(10)16-tetraene (ESE-16) was investigated in terms of its in vitro antiproliferative effects on the esophageal carcinoma SNO cell line at a concentration of 0.18 μM and an exposure time of 24 h. Polarization-optical differential interference contrast and triple fluorescent staining (propidium iodide, Hoechst 33342 and acridine orange) revealed a decrease in cell density, metaphase arrest, and the occurrence of apoptotic bodies in the ESE-16-treated cells when compared to relevant controls. Treated cells also showed an increase in the presence of acidic vacuoles and lysosomes, suggesting the occurrence of

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Keywords

Antiproliferative, ESE-16, Esophageal carcinoma, In vitro, Apoptosis, Autophagy, 2-ethyl-3-O-sulfamoyl-estra-1,3,5(10)16-tetraene

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Citation

Wolmarans, E, Mqoco, TV, Stander, A, Nkandeu, SD, Sippel, KH, Mckenna, R & Joubert, AM 2014, 'Novel estradiol analogue induces apoptosis and autophagy in esophageal carcinoma cells', Cellular & Molecular Biology Letters, vol. 19, no. 1, pp. 98-115.