Microsatellite and mating type primers for the maize and sorghum pathogen, Exserohilum turcicum

Show simple item record

dc.contributor.author Haasbroek, M.P.
dc.contributor.author Craven, M.
dc.contributor.author Barnes, Irene
dc.contributor.author Crampton, Bridget Genevieve
dc.date.accessioned 2015-03-05T09:19:52Z
dc.date.available 2015-03-05T09:19:52Z
dc.date.issued 2014-09
dc.description.abstract Northern corn leaf blight (NCLB) is a destructive foliar disease of maize that results from infection with the fungal pathogen, Exserohilum turcicum. Annual yield losses incurred from NCLB in South Africa may exceed 50 % when environmental conditions optimal for disease development prevail. In order to study the genetic diversity of E. turcicum, 13 microsatellite markers and mating type PCR primers were developed. Thirty-two primer pairs were designed from the E. turcicum genome sequence to flank microsatellite regions. A multiplex PCR assay amplifying both mating type idiomorphs was designed from the MAT1-1 and MAT1-2 gene sequences, and a protocol for multiplex PCR amplification of MAT loci was optimized. Initial screening identified 13 microsatellite regions that were polymorphic in 9 isolates of E. turcicum. To test the efficacy of the markers, 26 isolates of E. turcicum from 6 South African provinces, including 2 isolates from sorghum, were genotyped. A total of 90 alleles across 13 loci were obtained and the gene diversity ranged from 0.074 to 0.929. Cross-species amplification withE. rostratum was obtained for one SSR marker (SSR27). The MAT markers were specific to E. turcicum and could be used to differentiate isolates of E. turcicum and E. rostratum. The markers developed in this study will be useful to elucidate the population genetic structure, genetic diversity and mode of reproduction of E. turcicum on maize and sorghum. en_ZA
dc.description.embargo 2015-09-30 en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship Research supported in part by the National Research Foundation of South Africa (grant specific unique reference number (UID) 85076), and by the Maize Trust and the University of Pretoria’s Research and Development Programme. en_ZA
dc.description.uri http://link.springer.com/journal/13313 en_ZA
dc.identifier.citation Haasbroek, MP, Craven, M, Barnes, I & Crampton, BG 2014, 'Microsatellite and mating type primers for the maize and sorghum pathogen, Exserohilum turcicum', Australasian Plant Pathology, vol. 43, no. 5, pp. 577-581. en_ZA
dc.identifier.issn 0815-3191 (print)
dc.identifier.issn 1448-6032 (online)
dc.identifier.other 10.1007/s13313-014-0289-4
dc.identifier.uri http://hdl.handle.net/2263/43875
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Australasian Plant Pathology Society 2014. The original publication is available at : http://link.springer.comjournal/13313 en_ZA
dc.subject Fungi en_ZA
dc.subject Mating type markers en_ZA
dc.subject Microsatellite en_ZA
dc.subject Multiplex polymerase chain reaction (M-PCR) en_ZA
dc.subject Northern corn leaf blight (NCLB) en_ZA
dc.subject Setosphaeria turcica en_ZA
dc.title Microsatellite and mating type primers for the maize and sorghum pathogen, Exserohilum turcicum en_ZA
dc.type Postprint Article en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record