Antioxidant supplementation can reduce the survival costs of excess amino acid intake in honeybees

Show simple item record Archer, C. Ruth Kohler, Angela Pirk, Christian Walter Werner Oosthuizen, Vinette Apostolides, Zeno Nicolson, Sue W. 2015-02-05T11:04:30Z 2015-02-05T11:04:30Z 2014-12
dc.description.abstract Over-consuming amino acids is associated with reduced survival in many species, including honeybees. The mechanisms responsible for this are unclear but one possibility is that excessive intake of amino acids increases oxidative damage. If this is the case, antioxidant supplementation may help reduce the survival costs of high amino acid intake. We tested this hypothesis in African honeybees (Apis mellifera scutellata) using the major antioxidant in green tea, epigallocatechin-3-gallate (EGCG). We first determined the dose-range of EGCG that improved survival of caged honeybees fed sucrose solution. We then provided bees with eight diets that differed in their ratio of essential amino acids (EAA) to carbohydrate (C) (0:1, 1:250, 1:100, 1:75, 1:50, 1:25, 1:10, 1:5 EAA:C) and also in their EGCG dose (0.0 or 0.4 mM). We found that bees fed sucrose only solution survived better than bees fed EAA diets. Despite this, bees preferred a diet that contained intermediate ratios of EAA:C (ca. 1:25), which may represent the high demands for nitrogen of developing nurse bees. EGCG supplementation improved honeybee survival but only at an intermediate dose (0.3–0.5 mM) and in bees fed low EAA diets (1:250, 1:100 EAA:C). That EGCG counteracted the lifespan reducing effects of eating low EAA diets suggests that oxidative damage may be involved in the association between EAAs and lifespan in honeybees. However, that EGCG had no effect on survival in bees fed high EAA diets suggests that there are other physiological costs of over-consuming EAAs in honeybees. en_ZA
dc.description.librarian hj2015 en_ZA
dc.description.sponsorship A grant from the BBSRC, NERC, the Wellcome Trust, Defra, and the Scottish Government under the Insect Pollinators Initiative (BB/I000968/1). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Archer, CR, Kohler, A, Pirk, CWW, Oosthuizen, V, Apostolides, Z & Nicolson, SW 2014, 'Antioxidant supplementation can reduce the survival costs of excess amino acid intake in honeybees', Journal of Insect Physiology, vol. 71, pp. 78-86. en_ZA
dc.identifier.issn 0022-1910 (print)
dc.identifier.issn 1879-1611 (online)
dc.identifier.other 10.1016/j.jinsphys.2014.10.006
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2014 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Insect Physiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Insect Physiology, vol. 71, pp. 78-86, 2014. doi : 10.1016/j.jinsphys.2014.10.006 en_ZA
dc.subject Carbohydrate en_ZA
dc.subject Essential amino acids (EAA) en_ZA
dc.subject Intake array en_ZA
dc.subject Epigallocatechin-3-gallate (EGCG) en_ZA
dc.subject African honeybees (Apis mellifera scutellata) en_ZA
dc.title Antioxidant supplementation can reduce the survival costs of excess amino acid intake in honeybees en_ZA
dc.type Postprint Article en_ZA

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