First molecular assessment of the African swine fever virus status of Ornithodoros ticks from Swaziland

Show simple item record Boshoff, Carin I. Bastos, Armanda D.S. Dube, Mzwandi M. Heath, Livio Edward 2015-01-23T08:54:12Z 2015-01-23T08:54:12Z 2014-12
dc.description.abstract African swine fever (ASF) is an economically significant haemorrhagic disease of domestic pigs. It is caused by the African swine fever virus (ASFV), a deoxyribonucleic acid (DNA) arbovirus. Argasid ticks of the genus Ornithodoros, which are widely distributed throughout southern Africa, play a primary role in virus maintenance and spread within the endemic sylvatic cycle. The ASF status of Swaziland is unknown, but this land-locked country is surrounded by ASF-positive countries, has a burgeoning pig industry and sylvatic cycle hosts present within its borders. In this first assessment of ASF status, warthog burrows in seven nature reserves and game management areas in Swaziland were investigated for tick and virus presence. Tick infestation rates of between 33.3% – 88.8% were recovered for the four Ornithodoros-infested reserves. A total of 562 ticks were screened for virus genome presence using a duplex Polymerase Chain Reaction (PCR) that targets the C-terminal end of the p72 gene of the ASFV and confirms DNA integrity through amplification of the 16S rRNA tick host gene. All samples were negative for virus genome presence and positive for the tick genome target. Nucleotide sequencing of the latter confirmed that Ornithodoros ticks from Swaziland are identical to those from the Kruger National Park in South Africa across the gene region characterised. Whilst this first evaluation of ASF presence in Swaziland indicates that the virus does not appear to be present in the key virus vector, the presence of sylvatic cycle hosts, together with the country’s proximity to ASF-affected countries calls for expanded investigations and regular monitoring of the ASF status of Swaziland. en_ZA
dc.description.librarian tm2015 en_ZA
dc.description.sponsorship Agricultural Research Council-Onderstepoort Veterinary Institute en_ZA
dc.description.uri en_ZA
dc.identifier.citation Boshoff, C.I., Bastos, A.D.S., Dube, M.M. & Heath, L., 2014, ‘First molecular assessment of the African swine fever virus status of Ornithodoros ticks from Swaziland’, Onderstepoort Journal of Veterinary Research 81(1), Art. #846, 5 pages. http://dx.doi. org/10.4102/ojvr.v81i1.846. en_ZA
dc.identifier.issn 2219-0635
dc.identifier.issn 0030-2465
dc.identifier.other 10.4102/ojvr.v81i1.846
dc.language.iso en en_ZA
dc.publisher AOSIS OpenJournals Publishing en_ZA
dc.rights © 2014. The Authors. Licensee: AOSIS OpenJournals. This work is licensed under the Creative Commons Attribution License en_ZA
dc.subject African swine fever (ASF) en_ZA
dc.subject Ornithodoros ticks en_ZA
dc.subject Polymerase chain reaction (PCR) en_ZA
dc.subject Deoxyribonucleic acid (DNA) en_ZA
dc.subject Swaziland en_ZA
dc.title First molecular assessment of the African swine fever virus status of Ornithodoros ticks from Swaziland en_ZA
dc.type Article en_ZA

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