A single dilution enzyme-linked immunosorbent assay (ELISA), utilizing a reference serum with a known,
predetermined end-titre was used to compute antibody titres of serum samples against African horsesickness
virus. Compared to the complement fixation test it was found to be highly reproducible and sensitive. The assay
also reduced the effect of between-test variations on test results and proved superior to an existing single-dilution
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