Analysis of microsatellite markers in the genome of the plant pathogen Ceratocystis fimbriata

Show simple item record Simpson, M.C. (Melissa Claire) Wilken, Pieter Markus Coetzee, Martin Petrus Albertus Wingfield, Michael J. Wingfield, Brenda D. 2014-11-07T12:50:00Z 2014-11-07T12:50:00Z 2013-07
dc.description.abstract Ceratocystis fimbriata sensu lato represents a complex of cryptic and commonly plant pathogenic species that are morphologically similar. Species in this complex have been described using morphological characteristics, intersterility tests and phylogenetics. Microsatellite markers have been useful to study the population structure and origin of some species in the complex. In this study we sequenced the genome of C. fimbriata. This provided an opportunity to mine the genome for microsatellites, to develop new microsatellite markers, and map previously developed markers onto the genome. Over 6000 microsatellites were identified in the genome and their abundance and distribution was determined. Ceratocystis fimbriata has a medium level of microsatellite density and slightly smaller genome when compared with other fungi for which similar microsatellite analyses have been performed. This is the first report of a microsatellite analysis conducted on a genome sequence of a fungal species in the order Microascales. Forty-seven microsatellite markers have been published for population genetic studies, of which 35 could be mapped onto the C. fimbriata genome sequence. We developed an additional ten microsatellite markers within putative genes to differentiate between species in the C. fimbriata s.l. complex. These markers were used to distinguish between 12 species in the complex. en_US
dc.description.librarian hj2014 en_US
dc.description.sponsorship The National Research Foundation (NRF), the South African Biosystematics Initiative (SABI), members of the Tree Protection Co-Operative Programme (TPCP), and the Department of Science and Technology (DST)/ NRF Centre of Excellence in Tree Health Biotechnology (CTHB), South Africa. en_US
dc.description.uri en_US
dc.identifier.citation Simpson, MC, Wilken, PM, Coetzee, MPA, Wingfield, MJ & Wingfield BD 2013, 'Analysis of microsatellite markers in the genome of the plant pathogen Ceratocystis fimbriata', Fungal Biology, vol. 117, no. 7-8, pp. 545-555. en_US
dc.identifier.issn 1878-6162 (online)
dc.identifier.issn 1878-6146 (print)
dc.identifier.other 10.1016/j.funbio.2013.06.004
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2013 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Fungal Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Biology, vol. 117, no. 7-8, pp. 545-555, 2013. doi : 10.1016/j.funbio.2013.06.004. en_US
dc.subject Ceratocystis fimbriata en_US
dc.subject Genome mining en_US
dc.subject Microsatellites en_US
dc.title Analysis of microsatellite markers in the genome of the plant pathogen Ceratocystis fimbriata en_US
dc.type Postprint Article en_US

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