Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

Loading...
Thumbnail Image

Authors

Lin, Yao-Cheng
Boone, Morgane
Meuris, Leander
Lemmens, Irma
Van Roy, Nadine
Soete, Arne
Reumers, Joke
Moisse, Matthieu
Plaisance, Stéphane
Drmanac, Radoje

Journal Title

Journal ISSN

Volume Title

Publisher

Natural Product

Abstract

The HEK293 human cell lineage is widely used in cell biology and biotechnology. We here use whole genome resequencing of six 293 cell lines to study the dynamics of this aneuploid genome in response to the manipulations used to generate common 293 cell derivatives, such as transformation and stable clone generation (293T); suspension growth adaptation (293S) and cytotoxic lectin selection (293SG). Remarkably, we observe that copy number alteration detection could identify the genomic region that enabled cell survival under selective conditions (i.c. ricin selection). Furthermore, we present methods to detect human/vector genome breakpoints and a user-friendly visualization tool for the 293 genome data. We also establish that the genome structure composition is in steady state for most of these cell lines when standard cell culturing conditions are used. This resource enables novel and more informed studies with 293 cells, and we will distribute the sequenced cell lines to this effect.

Description

Keywords

Genome dynamics, Cell biology manipulations, Human embryonic kidney (HEK) 293

Sustainable Development Goals

Citation

Lin, Y-C, Boone, M, Meuris, L, Lemmens, I, Van Roy, N, Soete, A, Reumers, J, Moisse, M, Plaisance, S, Drmanac, R, Chen, J, Speleman, F, Lambrechts, D, Van De Peer, Y, Tavernier, J & Callewaert, N 2014, 'Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations', Nature Communications, vol. 5, art. #4767, pp. 1-12.