Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations
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Date
Authors
Lin, Yao-Cheng
Boone, Morgane
Meuris, Leander
Lemmens, Irma
Van Roy, Nadine
Soete, Arne
Reumers, Joke
Moisse, Matthieu
Plaisance, Stéphane
Drmanac, Radoje
Journal Title
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Natural Product
Abstract
The HEK293 human cell lineage is widely used in cell biology and biotechnology. We here use whole
genome resequencing of six 293 cell lines to study the dynamics of this aneuploid genome in response
to the manipulations used to generate common 293 cell derivatives, such as transformation and stable
clone generation (293T); suspension growth adaptation (293S) and cytotoxic lectin selection (293SG).
Remarkably, we observe that copy number alteration detection could identify the genomic region that
enabled cell survival under selective conditions (i.c. ricin selection). Furthermore, we present methods
to detect human/vector genome breakpoints and a user-friendly visualization tool for the 293 genome
data. We also establish that the genome structure composition is in steady state for most of these cell
lines when standard cell culturing conditions are used. This resource enables novel and more informed
studies with 293 cells, and we will distribute the sequenced cell lines to this effect.
Description
Keywords
Genome dynamics, Cell biology manipulations, Human embryonic kidney (HEK) 293
Sustainable Development Goals
Citation
Lin, Y-C, Boone, M, Meuris, L, Lemmens, I, Van Roy, N, Soete, A, Reumers, J, Moisse, M, Plaisance, S, Drmanac, R, Chen, J, Speleman, F, Lambrechts, D, Van De Peer, Y, Tavernier, J & Callewaert, N 2014, 'Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations', Nature Communications, vol. 5, art. #4767, pp. 1-12.