We outline the experiences and the challenges of optimizing two-dimensional gas chromatography
coupled with time-of-flight mass spectrometry (GCxGC-TOF-MS) in conjunction with the H4IIE-luc bioassay
for analyzing polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans
(PCDFs) in the South African context. Investigating such alternative analytical methods can assist
countries with developing economies to meet their obligations under the Stockholm Convention.