Genome sequence analysis of in vitro and in vivo phenotypes of Bunyamwera and Ngari virus isolates from Northern Kenya

Show simple item record Odhiambo, Collins Venter, Marietjie Limbaso, Konongoi Swanepoel, Robert Sang, Rosemary
dc.contributor.editor Aguilar, Patricia V. 2014-10-13T10:30:37Z 2014-10-13T10:30:37Z 2014-08-25
dc.description.abstract Biological phenotypes of tri-segmented arboviruses display characteristics that map to mutation/s in the S, M or L segments of the genome. Plaque variants have been characterized for other viruses displaying varied phenotypes including attenuation in growth and/or pathogenesis. In order to characterize variants of Bunyamwera and Ngari viruses, we isolated individual plaque size variants; small plaque (SP) and large plaque (LP) and determined in vitro growth properties and in vivo pathogenesis in suckling mice. We performed gene sequencing to identify mutations that may be responsible for the observed phenotype. The LP generally replicated faster than the SP and the difference in growth rate was more pronounced in Bunyamwera virus isolates. Ngari virus isolates were more conserved with few point mutations compared to Bunyamwera virus isolates which displayed mutations in all three genome segments but majority were silent mutations. Contrary to expectation, the SP of Bunyamwera virus killed suckling mice significantly earlier than the LP. The LP attenuation may probably be due to a non-synonymous substitution (T858I) that mapped within the active site of the L protein. In this study, we identify natural mutations whose exact role in growth and pathogenesis need to be determined through site directed mutagenesis studies. en_US
dc.description.librarian am2014 en_US
dc.description.sponsorship The Swedish International Development Agency (SIDA) administered through the African Region Postgraduate Programme in Insect Science (ARPPIS) under Capacity Building of the International Centre of Insect Physiology and Ecology. en_US
dc.description.uri en_US
dc.identifier.citation Odhiambo C, Venter M, Limbaso K, Swanepoel R, Sang R (2014) Genome Sequence Analysis of In Vitro and In Vivo Phenotypes of Bunyamwera and Ngari Virus Isolates from Northern Kenya. PLoS ONE 9(8): e105446. DOI: 10.1371/journal.pone.0105446. en_US
dc.identifier.issn 1932-6203
dc.identifier.other 10.1371/journal.pone.0105446
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.rights This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. en_US
dc.subject Northern, Kenya en_US
dc.subject In vitro growth en_US
dc.subject In vivo pathogenesis en_US
dc.subject Gene sequencing en_US
dc.subject Ngari virus en_US
dc.subject Bunyamwera virus en_US
dc.title Genome sequence analysis of in vitro and in vivo phenotypes of Bunyamwera and Ngari virus isolates from Northern Kenya en_US
dc.type Article en_US

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