A selective procedure for the field isolation of pathogenic Streptococcus spp. of rainbow trout (Salmo gairdneri)
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Date
Authors
Bragg, R.R. (Robert Richard)
Todd, Jacqueline M.
Lordan, Shirley M.
Combrink, Marianne E.
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Government Printer, Pretoria.
Abstract
A procedure established for the selective isolation of the species of Streptococcus responsible for rainbow trout streptococcosis in South Africa, consisted of the inoculation of samples into nutrient broth which had been supplemented with 100 µg/ml of nalidixic acid, 160 µg/ml of oxolinic acid or 200 µg/ml of sodium azide. After incubation, the sample was plated onto tetrazolium agar on which the rainbow trout pathogenic Streptococcus species grew as a red colony. The colonies were isolated from the tetrazolium agar and identified as rainbow trout pathogenic isolates by biochemical and serological tests. In the laboratory the selective procedure is capable of detecting about 2 bacteria per ml. This procedure was used in the field and biochemically identical Streptococcus species were found in the mud and a freshwater crab from the water source of a site with a history of streptococcosis.
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Bragg, RR, Todd, JM, Lordan, SM & Combrink, ME 1989, 'A selective procedure for the field isolation of pathogenic Streptococcus spp. of rainbow trout (Salmo gairdneri)', Onderstepoort Journal of Veterinary Research, vol. 56, no. 3, pp. 179-184.