MAT gene idiomorphs suggest a heterothallic sexual cycle in a predominantly asexual and important pine pathogen

Show simple item record Bihon, Wubetu Wingfield, Michael J. Slippers, Bernard Duong, Tuan A. Wingfield, Brenda D. 2014-09-23T08:49:24Z 2014-09-23T08:49:24Z 2014-01
dc.description.abstract Diplodia pinea (=Sphaeropsis sapinea) is a well-known and economically important latent pathogen of Pinus spp. in many parts of the world. Despite intensive scrutiny, its sexual state has never been observed and the fungus has thus been considered exclusively asexual. It was, therefore, surprising that a recent population genetics study showed high genotypic diversity and random association of alleles in a number of populations, suggesting that the pathogen has a cryptic sexual stage. Using the genome sequence of two individual D. pinea isolates, we interrogated the structure of the MAT locus in this fungus. The results suggested that D. pinea is heterothallic (self-sterile) with complete and apparently functional copies of the MAT genes containing the a-1 and HMG domains present in different isolates. In addition to the MAT1-2-1 and MAT1-1-1 genes, we found a MAT1-1-4 gene in the MAT1-1 idiomorph and a novel MAT1-2-5 gene in the MAT1-2 idiomorph. Importantly, the frequencies of occurrence of both idiomorphs in populations examined were not significantly different from a 1:1 ratio, which would be expected in sexually reproducing populations. Although the sexual state has never been observed, the results strongly suggest that D. pinea has a cryptic, heterothallic sexual cycle. en_US
dc.description.librarian hb2014 en_US
dc.description.sponsorship Department of Science and Technology (DST)/National Research Foundation (NRF) Centre of Excellence in Tree Health Biotechnology, members of the Tree Protection Cooperative Program (TPCP) and Genomics Research Institute (University of Pretoria Institutional Research Theme).Claude Leon Foundation. en_US
dc.description.uri en_US
dc.identifier.citation Bihon, W, Wingfield, MJ, Slippers, B, Duong, TA & Wingfield, BD 2014, 'MAT gene idiomorphs suggest a heterothallic sexual cycle in a predominantly asexual and important pine pathogen', Fungal Genetics and Biology, vol. 62, pp. 55-61. en_US
dc.identifier.issn 1087-1845 (print)
dc.identifier.issn 1096-0937 (online)
dc.identifier.other 10.1016/j.fgb.2013.10.013
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2013 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Fungal Genetics and Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Genetics and Biology, vol. 62, pp. 55-61, 2014. doi : 10.1016/j.fgb.2013.10.013. en_US
dc.subject Diplodia pinea en_US
dc.subject Endophyte en_US
dc.subject Heterothallic en_US
dc.subject Mating type en_US
dc.subject Idiomorph en_US
dc.subject Cryptic sex en_US
dc.title MAT gene idiomorphs suggest a heterothallic sexual cycle in a predominantly asexual and important pine pathogen en_US
dc.type Postprint Article en_US

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