Characterisation and expression profile of the bovine cathelicidin gene repertoire in mammary tissue

Show simple item record Whelehan, Cormac J. Barry-Reidy, Anne Meade, Kieran G. Eckersall, P. David Chapwanya, Aspinas Narciandi, Fernando Lloyd, Andrew T. O’Farrelly, Cliona 2014-09-09T10:11:26Z 2014-09-09T10:11:26Z 2014-02-13
dc.description.abstract BACKGROUND: Cathelicidins comprise a major group of host-defence peptides. Conserved across a wide range of species, they have several functions related to host defence. Only one cathelicidin has been found in humans but several cathelicidin genes occur in the bovine genome. We propose that these molecules may have a protective role against mastitis. The aim of this study was to characterise the cathelicidin gene-cluster in the bovine genome and to identify sites of expression in the bovine mammary gland. RESULTS: Bioinformatic analysis of the bovine genome (BosTau7) revealed seven protein-coding cathelicidin genes, CATHL1-7, including two identical copies of CATHL4, as well as three additional putative cathelicidin genes, all clustered on the long arm of chromosome 22. Six of the seven protein-coding genes were expressed in leukocytes extracted from milk of high somatic cell count (SCC) cows. CATHL5 was expressed across several sites in the mammary gland, but did not increase in response to Staphylococcus aureus infection. CONCLUSIONS: Here, we characterise the bovine cathelicidin gene cluster and reconcile inconsistencies in the datasets of previous studies. Constitutive cathelicidin expression in the mammary gland suggests a possible role for these host defence peptides its protection. en_US
dc.description.librarian am2014 en_US
dc.description.sponsorship An Irish Department of Agriculture, Fisheries and Food Research Stimulus Fund Grant (RSF 06-340). en_US
dc.description.uri en_US
dc.identifier.citation Whelehan, CJ, Barry-Reidy, A, Meade, KG, Eckersall, PD, Chapwanya, A, Narciandi, F, Lloyd, AT & O'Farrelly, C, 'Characterisation and expression profile of the bovine cathelicidin gene repertoire in mammary tissue', BMC Genomics, vol. 15, art. 28, pp. 1-13. en_US
dc.identifier.issn 1471-2164
dc.identifier.other 10.1186/1471-2164-15-128
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2014 Whelehan et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. en_US
dc.subject Hidden Markov Model (HMM) en_US
dc.subject Gene cluster en_US
dc.subject Locus en_US
dc.subject Tissue expression en_US
dc.subject Ccathelicidin gene-cluster en_US
dc.subject Bovine genome en_US
dc.subject Bovine mammary gland en_US
dc.subject Cathelicidin en_US
dc.title Characterisation and expression profile of the bovine cathelicidin gene repertoire in mammary tissue en_US
dc.type Article en_US

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