Strict control measures apply to movement of buffalo in South Africa including testing for Theileria parva, the causative
agent of Corridor disease in cattle. The official test is a real-time hybridization PCR assay that amplifies the 18S rRNA V4
hyper-variable region of T. parva, T. sp. (buffalo) and T. sp. (bougasvlei). Mixed infections with the latter organisms affect
diagnostic sensitivity due to PCR suppression. While the incidence of mixed infections in the Corridor disease endemic
region of South Africa is significant, little information is available on the specific distribution and prevalence of T. sp.
(buffalo) and T. sp. (bougasvlei). Specific real-time PCR assays were developed and a total of 1211 samples known to
harbour these parasites were screened. Both parasites are widely distributed in southern Africa and the incidence of mixed
infections with T. parva within the endemic region is similar (25–50%). However, a significant discrepancy exists in
regard to mixed infections of T. sp. (buffalo) and T. sp. (bougasvlei) (10%). Evidence for speciation between T. sp.
(buffalo) and T. sp. (bougasvlei) is supported by phylogenetic analysis of the COI gene, and their designation as different
species. This suggests mutual exclusion of parasites and the possibility of hybrid sterility in cases of mixed infections.
Theileria parva is the causative agent of Corridor disease in cattle in South Africa. The African
buffalo (Syncerus caffer) is the reservoir host, and, as these animals are important for eco-tourism in
South Africa, it ...
The African buffalo (Syncerus caffer) is a natural reservoir host for both pathogenic and nonpathogenic
Theileria species. These often occur naturally as mixed infections in buffalo.
Although the benign and mildly ...
Pienaar, Ronel; Potgieter, Fred T.; Latif, Abdalla A.; Thekisoe, Oriel M.M.; Mans, Ben J. (Barend Johannes)(Cambridge University Press, 2011-02-11)
Buffalo-adapted Theileria parva causes Corridor disease in cattle. Strict control measures therefore apply to the movement
of buffalo in South Africa and include mandatory testing of buffalo for the presence of T. parva. ...