The Brugia malayi neuropeptide receptor-4 is activated by FMRFamide-like peptides and signals via Gα

Show simple item record Anderson, Ross C. Newton, Claire L. Millar, Robert P. Katz, Arieh A. 2014-08-25T11:32:38Z 2014-08-25T11:32:38Z 2014-06
dc.description.abstract Genetic studies undertaken in the model organism Caenorhabditis elegans have demonstrated the importance of neuropeptidergic signalling in nematode physiology. Disruption of this signalling may have deleterious phenotypic consequences, including altered locomotion, feeding behaviour, and reproduction. Neuropeptide G protein-coupled receptors (GPCRs) that transduce many of these signals therefore represent cogent drug targets. Recently published genomic sequencing data for a number of parasitic helminths of medical and veterinary importance has revealed the apparent conservation of a number of neuropeptides, and neuropeptide receptors between parasitic and free-living species, raising the intriguing possibility of developing broad-spectrum anthelmintic therapeutics. Here, we identify and clone a neuropeptide receptor, NPR-4, from the human filarial nematode Brugia malayi and demonstrate its activation in vitro, by FMRFamide-like peptides of the FLP-18 family, and intracellular signalling via G i mediated pathways. These data represent the first example of deorphanisation of a neuropeptide GPCR in any parasitic helminth species. en_US
dc.description.department Zoology and Entomology
dc.description.department Mammal Research Institute
dc.description.librarian hb2014 en_US
dc.description.sponsorship MRC South Africa and the University of Cape Town. en_US
dc.description.uri en_US
dc.identifier.citation Anderson, RC, Newton, CL, Millar, RP & Katz, AA 2014, 'The Brugia malayi neuropeptide receptor-4 is activated by FMRFamide-like peptides and signals via Gα', Molecular and Biochemical Parasitology, vol. 195, no. 1, pp. 54-58. en_US
dc.identifier.issn 0166-6851 (print)
dc.identifier.issn 1872-9428 (online)
dc.identifier.other 10.1016/j.molbiopara.2014.07.002
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2014 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Molecular and Biochemical Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Molecular and Biochemical Parasitology, vol. 195, no.1, pp. 54-58, 2014. doi : 10.1016/j.molbiopara.2014.07.002. en_US
dc.subject Brugia malayi en_US
dc.subject FMRFamide-like peptides en_US
dc.subject Neuropeptide en_US
dc.subject Neuropeptide receptor en_US
dc.subject Lymphatic filariasis (LF) en_US
dc.subject G protein-coupled receptors (GPCRs) en_US
dc.title The Brugia malayi neuropeptide receptor-4 is activated by FMRFamide-like peptides and signals via Gα en_US
dc.type Postprint Article en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record