Abstract:
Transcriptome analysis through next-generation sequencing technologies allows the generation of detailed gene
catalogs for non-model species, at the cost of new challenges with regards to computational requirements and
bioinformatics expertise. Here, we present TRAPID, an online tool for the fast and efficient processing of assembled
RNA-Seq transcriptome data, developed to mitigate these challenges. TRAPID offers high-throughput open reading
frame detection, frameshift correction and includes a functional, comparative and phylogenetic toolbox, making use
of 175 reference proteomes. Benchmarking and comparison against state-of-the-art transcript analysis tools reveals
the efficiency and unique features of the TRAPID system. TRAPID is freely available at http://bioinformatics.psb.
ugent.be/webtools/trapid/.
