Peste des petits ruminants virus tissue tropism and pathogenesis in sheep and goats following experimental infection

Show simple item record Truong, Thang Boshra, Hani Embury-Hyatt, Carissa Nfon, Charles Gerdts, Volker Tikoo, Suresh Babiuk, Lorne A. Kara, Pravesh Deepak Chetty, Thireshni Mather, Arshad Wallace, David Brian Babiuk, Shawn 2014-08-18T10:12:38Z 2014-08-18T10:12:38Z 2014-01-30
dc.description.abstract Peste des petits ruminants (PPR) is a viral disease which primarily affects small ruminants, causing significant economic losses for the livestock industry in developing countries. It is endemic in Saharan and sub-Saharan Africa, the Middle East and the Indian sub-continent. The primary hosts for peste des petits ruminants virus (PPRV) are goats and sheep; however recent models studying the pathology, disease progression and viremia of PPRV have focused primarily on goat models. This study evaluates the tissue tropism and pathogenesis of PPR following experimental infection of sheep and goats using a quantitative time-course study. Upon infection with a virulent strain of PPRV, both sheep and goats developed clinical signs and lesions typical of PPR, although sheep displayed milder clinical disease compared to goats. Tissue tropism of PPRV was evaluated by real-time RT-PCR and immunohistochemistry. Lymph nodes, lymphoid tissue and digestive tract organs were the predominant sites of virus replication. The results presented in this study provide models for the comparative evaluation of PPRV pathogenesis and tissue tropism in both sheep and goats. These models are suitable for the establishment of experimental parameters necessary for the evaluation of vaccines, as well as further studies into PPRV-host interactions. en_US
dc.description.librarian am2014 en_US
dc.description.sponsorship A Canadian International Food Security Research Fund (CIFSRF) grant (no. 106930: Livestock vaccines against viral diseases for sub-Saharan Africa) by the Canadian International Development Research Centre (IDRC) and Canadian International Development Agency (CIDA). en_US
dc.description.uri en_US
dc.identifier.citation Truong T, Boshra H, Embury-Hyatt C, Nfon C, Gerdts V, et al. (2014) Peste des Petits Ruminants Virus Tissue Tropism and Pathogenesis in Sheep and Goats following Experimental Infection. PLoS ONE 9(1): e87145. DOI: 10.1371/journal.pone.0087145. en_US
dc.identifier.issn 1932-6203
dc.identifier.other 10.1371/journal.pone.0087145
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2014 Truong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License. en_US
dc.subject Livestock industry en_US
dc.subject Goats en_US
dc.subject Sheep en_US
dc.subject Peste des petits ruminants (PPR) en_US
dc.subject Viral disease en_US
dc.title Peste des petits ruminants virus tissue tropism and pathogenesis in sheep and goats following experimental infection en_US
dc.type Article en_US

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