Laboratory confirmation of African horsesickness in the Western Cape : application of a F(ab')₂-based indirect ELISA

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dc.contributor.author Du Plessis, D.H.
dc.contributor.author Van Wyngaardt, W.
dc.contributor.author Gerdes, Gertruida Hermanna
dc.contributor.author Opperman, E.
dc.contributor.editor Verwoerd, Daniel Wynand
dc.date.accessioned 2014-08-18T06:28:07Z
dc.date.available 2014-08-18T06:28:07Z
dc.date.created 2013
dc.date.issued 1991
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en
dc.description.abstract Recently a suspected outbreak of African horsesickness in the Western Cape Province resulted in the deaths of four foals and one adult horse. Spleen samples from these animals were subjected to analysis by an enzyme-linked immunosorbent assay (ELISA) which uses F(ab') ₂ fragments of immunoglobulins to detect African horse sickness virus (AHSV) antigens. The results of the immunoassay were compared with those obtained by isolation followed by serotyping as is currently applied by the Reference Centre at the Veterinary Research Institute, Onderstepoort. Samples of spleen tissue from the four foals contained sufficient antigen to be readily detectable by ELISA. A marginally positive signal was obtained with the tissue from the adult horse. This sample was inoculated onto VERO cells and four days were allowed for viral multiplication. Subsequently, when the cell culture was assayed by F(ab')₂ -ELISA, a much higher absorbance value than that obtained with the original spleen sample resulted, thus confirming the presence of AHSV in the initial specimen. The F(ab')₂-ELISA has potential to be used as an initial diagnostic test to screen for AHSV. en
dc.description.librarian mn2014
dc.identifier.citation Du Plessis, DH, Van Wyngaardt, W, Gerdes, GH & Opperman, E 1991, 'Laboratory confirmation of African horsesickness in the Western Cape : application of a F(ab')₂-based indirect ELISA', Onderstepoort Journal of Veterinary Research, vol. 58, no. 1, pp. 1-3. en
dc.identifier.issn 0330-2465
dc.identifier.uri http://hdl.handle.net/2263/41352
dc.language.iso en en
dc.publisher Published by the Agricultural Research Council, Onderstepoort Veterinary Institute. en
dc.rights ©South Africa. Dept. of Agricultural Technical Services. ©University of Pretoria. Dept. of Library Services (digital). en
dc.subject Veterinary medicine en
dc.subject.lcsh Veterinary medicine -- South Africa
dc.subject.lcsh African horse sickness -- South Africa en
dc.title Laboratory confirmation of African horsesickness in the Western Cape : application of a F(ab')₂-based indirect ELISA en
dc.type Article en


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