Genetic variants of APOC3 promoter and HLA-B genes in an HIV infected cohort in Northern South Africa : a pilot study
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Date
Authors
Masebe, Tracy
Bessong, Pascal Obong
Ndip Ndip, Roland
Meyer, Debra
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI Publishing
Abstract
Metabolic disorders and hypersensitivities affect tolerability and impact
adherence to highly active antiretroviral therapy (HAART). The aim of this study was to
determine the prevalence of C-482T/T-455C variants in the Apolipoprotein C3 (APOC3)
promoter gene and Human leukocyte antigen (HLA)-B*57:01, known to impact lipid
metabolic disorders and hypersensitivity respectively; and to correlate genotypes with
gender, CD4+ cell count and viral load in an HIV infected cohort in northern South Africa.
Frequencies of C-482 and T-455 polymorphisms in APOC3 were determined by restriction
fragment length polymorphism analysis. Allele determination for HLA-B was performed
with Assign SBT software in an HLA library. Analysis of APOC3 C-482 site revealed a
prevalence of 196/199 (98.5%) for CC, 1/199 (0.5%) for CT and 2/199 (1.0%) for TT
genotype (p = 0.000 with 1° of freedom; χ2 = 126.551). For the T-455 site, prevalences
were: 69/199 (35%) for TT and 130/199 (65%) for the CC genotype (p = 0.000 with 1° of
freedom; χ2 = 199). There was no association between gender and the presence of −482
(p = 1; χ2 = 0.00001) or −455 genotypes (p = 0.1628; χ2 = 1.9842). There was no significant
difference in the increase in CD4+ cell count irrespective of genotypes. Significant
increases in CD4+ cell count were observed in males and females considering the −455C genotype, but not in males for the −455T genotype. Viral load decreases were significant
with the −455C and −482C genotypes irrespective of gender. HLA-B*57:01 was not
identified in the study cohort. The apparently high prevalence of APOC3 T-455CC
genotype needs confirmation with a larger samples size and triglyceride measurements to
support screening of patients to pre-empt HAART associated lipid disorders.
Description
Keywords
Genetic variants, Highly active antiretroviral therapy (HAART), Lipid disorders, Apolipoprotein C3 (APOC3), Human leukocyte antigen (HLA), South Africa (SA)
Sustainable Development Goals
Citation
Masebe, T, Bessong, PO, Ndip Ndip, R & Meyer, D 2014, 'Genetic variants of APOC3 promoter and HLA-B genes in an HIV infected cohort in Northern South Africa : a pilot study', International Journal of Molecular Sciences, no. 15, pp. 11403-11415.