Genetic variants of APOC3 promoter and HLA-B genes in an HIV infected cohort in Northern South Africa : a pilot study

Loading...
Thumbnail Image

Authors

Masebe, Tracy
Bessong, Pascal Obong
Ndip Ndip, Roland
Meyer, Debra

Journal Title

Journal ISSN

Volume Title

Publisher

MDPI Publishing

Abstract

Metabolic disorders and hypersensitivities affect tolerability and impact adherence to highly active antiretroviral therapy (HAART). The aim of this study was to determine the prevalence of C-482T/T-455C variants in the Apolipoprotein C3 (APOC3) promoter gene and Human leukocyte antigen (HLA)-B*57:01, known to impact lipid metabolic disorders and hypersensitivity respectively; and to correlate genotypes with gender, CD4+ cell count and viral load in an HIV infected cohort in northern South Africa. Frequencies of C-482 and T-455 polymorphisms in APOC3 were determined by restriction fragment length polymorphism analysis. Allele determination for HLA-B was performed with Assign SBT software in an HLA library. Analysis of APOC3 C-482 site revealed a prevalence of 196/199 (98.5%) for CC, 1/199 (0.5%) for CT and 2/199 (1.0%) for TT genotype (p = 0.000 with 1° of freedom; χ2 = 126.551). For the T-455 site, prevalences were: 69/199 (35%) for TT and 130/199 (65%) for the CC genotype (p = 0.000 with 1° of freedom; χ2 = 199). There was no association between gender and the presence of −482 (p = 1; χ2 = 0.00001) or −455 genotypes (p = 0.1628; χ2 = 1.9842). There was no significant difference in the increase in CD4+ cell count irrespective of genotypes. Significant increases in CD4+ cell count were observed in males and females considering the −455C genotype, but not in males for the −455T genotype. Viral load decreases were significant with the −455C and −482C genotypes irrespective of gender. HLA-B*57:01 was not identified in the study cohort. The apparently high prevalence of APOC3 T-455CC genotype needs confirmation with a larger samples size and triglyceride measurements to support screening of patients to pre-empt HAART associated lipid disorders.

Description

Keywords

Genetic variants, Highly active antiretroviral therapy (HAART), Lipid disorders, Apolipoprotein C3 (APOC3), Human leukocyte antigen (HLA), South Africa (SA)

Sustainable Development Goals

Citation

Masebe, T, Bessong, PO, Ndip Ndip, R & Meyer, D 2014, 'Genetic variants of APOC3 promoter and HLA-B genes in an HIV infected cohort in Northern South Africa : a pilot study', International Journal of Molecular Sciences, no. 15, pp. 11403-11415.