Abstract:
BACKGROUND: The Fabaceae family is the second largest family of medicinal plants, containing more than 490
species which are being used as traditional medicine. The aim of this study was to determine the antioxidant
and antibacterial activity as well as the cytotoxicity of acetone leaf extracts of nine tree species from the
Fabaceae family that have not been investigated well previously for possible use in animal health and
production.
METHODS: The antibacterial activity was determined by a serial microdilution method against three Gram-positive and
three Gram-negative bacteria. Antioxidant activity was determined using free-radical scavenging assays. The safety of
the extracts was ascertained using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on Vero
African green monkey kidney cells.
RESULTS: Six of the nine acetone extracts had significant antibacterial activity against at least one of the six bacterial
species with (MIC 20–80 μg/mL). The Crotalaria capensis extract had the highest activity against Salmonella
typhimurium, followed by Indigofera cylindrica with MICs of 20 μg/mL and 40 μg/mL respectively. The Dalbergia nitidula
extract had free radical scavenging capacity (IC50 of 9.31 ± 2.14 μg/mL) close to that of the positive control Trolox in the
DPPH assay. The Xylia torreana extract also had high activity (IC50 of 14.56 ± 3.96 μg/mL) in the ABTS assay. There was a
good correlation between antioxidant activity and total phenolic content (R2 values > 0.8). The extracts had weak or no
toxicity to Vero cells, compared to the positive control doxorubicin with the LC50 varying from 10.70 ± 3.47 to 131.98 ±
24.87 μg/mL at the concentrations tested.
CONCLUSION: Extracts of D. nitidula, X. torreana, C. capensis and I. cylindrica had a low cytotoxicity and high antimicrobial
and/or antioxidant activity. These species are therefore promising candidates for the development of useful
antimicrobial/antioxidant preparations with a low cytotoxicity that may be useful in promoting animal health and
productivity.
