Antibacterial constituents of three Cameroonian medicinal plants : Garcinia nobilis, Oricia suaveolens and Balsamocitrus camerunensis

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dc.contributor.author Fouotsa, Hugues
dc.contributor.author Mbaveng, Armelle T.
dc.contributor.author Mbazoa, Celine D.
dc.contributor.author Nkengfack, Augustin E.
dc.contributor.author Farzana, Shaheen
dc.contributor.author Iqbal, Choudhary M.
dc.contributor.author Meyer, J.J.M. (Jacobus Johannes Marion)
dc.contributor.author Lall, Namrita
dc.contributor.author Kuete, Victor
dc.date.accessioned 2014-07-09T07:44:40Z
dc.date.available 2014-07-09T07:44:40Z
dc.date.issued 2013-04-10
dc.description.abstract BACKGROUND: Multidrug resistance is a worrying cause of treatment failure in bacterial infections. The search of bioactive constituents from medicinal plants against multidrug resistant (MDR) bacteria has significantly evolved in the two last decades. In the present study, twenty-two compounds (three terpenoids, eleven phenolics and eight alkaloids) isolated from three Cameroonian medicinal plants, namely Garcinia nobilis, Oricia suaveolens and Balsamocitrus camerunensis, as well as the crude extracts were tested for their antibacterial activities against Mycobacterium tuberculosis and Gram-negative bacteria amongst which were MDR active efflux pumps expressing phenotypes. METHODS: The microplate alamar blue assay (MABA) and the broth microdilution methods were used to determine the minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) of the studied samples. RESULTS: The results of the MIC determinations indicate that, the best crude extract was that from G. nobilis (GNB), its inhibitory effects being noted against 12 of the 14 tested bacteria. The extract of GNB also exhibited better anti-tuberculosis (MIC of 128 μg/ml M. tuberculosis against ATCC 27294 strain) and antibacterial (MIC of 64 μg/ml against Escherichia coli ATCC10536) activities compared to the extracts of O. suaveolens and B. camerunensis. Interestingly, 4-prenyl-2-(3,7-dimethyl-2,6-octadienyl)-1,3,5,8-tetrahydroxyxanthone (2), isolated from the most active extract GNB, also showed the best activity amongst compounds, inhibiting the growth of all the fourteen tested microorganisms. The lowest MIC value obtained with compound 2 was 8 μg/ml against M. tuberculosis ATCC 27294 and M. tuberculosis clinical MTCS2 strains. Other compounds showed selective activities with 11 of the 14 tested bacteria being sensitive to the xanthone, morusignin I (5) and the alkaloid, kokusaginine (13). CONCLUSIONS: The results of the present investigation provide evidence that the crude extract from G. nobilis, O. suaveolens and B. camerunensis as well as some of their compounds, and mostly compound 2 (isolated from G. nobilis,) could be considered as interesting natural antibacterial products. en_US
dc.description.librarian am2014 en_US
dc.description.sponsorship International Foundation for Science (IFS-Grant F/4579-2 to VK), TWAS for financial support for ICCBSTWAS fellowship at the H.E.J. Research Institute of Chemistry, International Center of Chemical Sciences, University of Karachi, Pakistan on year 2010 (HF). en_US
dc.description.uri http://www.biomedcentral.com/bmccomplementalternmed en_US
dc.identifier.citation Fouotsa, H, Mbaveng, AT, Mbazoa, CD, Nkengfack, AE, Farzana, S, Iqbal, CM, Meyer, JJM, Lall, N & Kuete, V 2013, 'Antibacterial constituents of three Cameroonian medicinal plants : Garcinia nobilis, Oricia suaveolens and Balsamocitrus camerunensis', BMC Complementary and Alternative Medicine, vol. 13, art. 81, pp. 1-10. en_US
dc.identifier.issn 1472-6882
dc.identifier.other 10.1186/1472-6882-13-81
dc.identifier.uri http://hdl.handle.net/2263/40676
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.rights © 2013 Fouotsa et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_US
dc.subject Antimycobacterial en_US
dc.subject Antibacterial en_US
dc.subject Cameroon en_US
dc.subject Compounds en_US
dc.subject Garcinia nobilis en_US
dc.subject Oricia suaveolens en_US
dc.subject Balsamocitrus camerunensis en_US
dc.title Antibacterial constituents of three Cameroonian medicinal plants : Garcinia nobilis, Oricia suaveolens and Balsamocitrus camerunensis en_US
dc.type Article en_US


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