Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants

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dc.contributor.advisor Kotze, J.M.
dc.contributor.coadvisor Verschoor, J.A. (Jan Adrianus), 1953-
dc.contributor.postgraduate Sanders, Gina Mercia
dc.date.accessioned 2014-06-17T12:59:50Z
dc.date.available 2014-06-17T12:59:50Z
dc.date.created 1993-05-30
dc.date.issued 2013 en_US
dc.description Dissertation (MSc Agric)--University of Pretoria, 1993.
dc.description.abstract The yearly losses incurred by bacterial blackspot disease are high. Often trees are asymptomatic, with the pathogen either in the resident phase or latent stage of infection. Detection of the pathogen in these asymptomatic trees is one of the most important means of controlling the disease. Isolates which consistently differed in virulence were isolated from symptomatic mango plants. These isolates could be categorised into four groups based upon differences in virulence. Monoclonal antibodies (mAbs) were successfully raised using separate and pooled isolates for immunisation. MAbsraised were of the lgG class and reacted with a proteinaceous epitope. These monoclonal antibodies could distinguish between different virulence groups of Xanthomonas campestris pv. mangiferaeindicae by means of Western Blot analysis. These antibodies were used along with a selective medium, BVGA for detection of epiphytic populations as well as latent infections in mango. An enrichment step prior to the enzyme- linked immunosorbent assay (ELISA) is important, since bacterial counts on trees with latent infections are too low to result in a positive signal. These techniques in combination are thus useful for detection and monitoring of the pathogen, which may play an important role in controlling the spread of the disease.
dc.description.availability unrestricted en_US
dc.description.department Microbiology and Plant Pathology en_US
dc.description.librarian gm2013 en_US
dc.identifier.citation Sanders, GM 1993, Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants, MSc (Agric) dissertation, University of Pretoria, Pretoria, viewed yymmdd<http://hdl.handle.net/2263/40197> en_US
dc.identifier.uri http://hdl.handle.net/2263/40197
dc.language.iso en en_US
dc.publisher University of Pretoria
dc.rights © 1993 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. en_US
dc.subject Mangiferaeindicae in mango plants en_US
dc.subject Xanthomonas campestris en_US
dc.subject UCTD
dc.title Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants en_US
dc.type Dissertation en_US


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