Molecular detection of Rickettsia felis and Candidatus Rickettsia asemboensis in fleas from human habitats, Asembo, Kenya

Show simple item record Jiang, Ju Maina, Alice N. Knobel, Darryn Leslie Cleaveland, Sarah Laudisoit, Anne Wamburu, Kabura Ogola, Eric Parola, Philippe Breiman, Robert F. Njenga, M. Kariuki Richards, Allen L. 2014-05-23T10:19:19Z 2014-05-23T10:19:19Z 2013-08
dc.description.abstract The flea-borne rickettsioses murine typhus (Rickettsia typhi) and flea-borne spotted fever (FBSF) (Rickettsia felis) are febrile diseases distributed among humans worldwide. Murine typhus has been known to be endemic to Kenya since the 1950s, but FBSF was only recently documented in northeastern (2010) and western (2012) Kenya. To characterize the potential exposure of humans in Kenya to flea-borne rickettsioses, a total of 330 fleas (134 pools) including 5 species (Xenopsylla cheopis, Ctenocephalides felis, Ctenocephalides canis, Pulex irritans, and Echidnophaga gallinacea) were collected from domestic and peridomestic animals and from human dwellings within Asembo, western Kenya. DNA was extracted from the 134 pooled flea samples and 89 (66.4%) pools tested positively for rickettsial DNA by 2 genus-specific quantitative real-time PCR (qPCR) assays based upon the citrate synthase (gltA) and 17-kD antigen genes and the Rfelis qPCR assay. Sequences from the 17-kD antigen gene, the outer membrane protein (omp)B, and 2 R. felis plasmid genes (pRF and pRFd) of 12 selected rickettsiapositive samples revealed a unique Rickettsia sp. (n = 11) and R. felis (n = 1). Depiction of the new rickettsia by multilocus sequence typing (MLST) targeting the 16S rRNA (rrs), 17-kD antigen gene, gltA, ompA, ompB, and surface cell antigen 4 (sca4), shows that it is most closely related to R. felis but genetically dissimilar enough to be considered a separate species provisionally named Candidatus Rickettsia asemboensis. Subsequently, 81 of the 134 (60.4%) flea pools tested positively for Candidatus Rickettsia asemboensis by a newly developed agentspecific qPCR assay, Rasemb. R. felis was identified in 9 of the 134 (6.7%) flea pools, and R. typhi the causative agent of murine typhus was not detected in any of 78 rickettsia-positive pools assessed using a species-specific qPCR assay, Rtyph. Two pools were found to contain both R. felis and Candidatus Rickettsia asemboensis DNA and 1 pool contained an agent, which is potentially new. en
dc.description.librarian hb2014 en
dc.description.librarian ab2014
dc.description.sponsorship Global Emerging Infections Surveillance and Response System, a Division of the Armed Forces Health Surveillance Center; work unit number 0000188M.0931.001.A0074 and the Wellcome Trust (grant no.81828). en
dc.description.uri en
dc.identifier.citation Jiang, J, Maina, AN, Knobel, DL, Cleaveland, S, Laudisoit, A, Wamburu, K, Ogola, E, Parola, P, Breiman, RF, Njenga, MK & Richards, AL 2013, 'Molecular detection of Rickettsia felis and Candidatus Rickettsia asemboensis in fleas from human habitats, Asembo, Kenya', Vector-Borne and Zoonotic Diseases, vol. 13, no. 8, pp. 550-558. en
dc.identifier.issn 1530-3667 (print)
dc.identifier.issn 1557-7759 (online)
dc.identifier.other 10.1089/vbz.2012.1123
dc.identifier.other 6602518021
dc.identifier.other O-7057-2014
dc.identifier.other 0000-0002-0425-3799
dc.language.iso en en
dc.publisher Mary Ann Liebert en
dc.relation.requires Adobe Acrobat Reader en
dc.rights © Mary Ann Liebert, Inc. en
dc.subject Fleas en
dc.subject PCR en
dc.subject Multilocus sequence typing en
dc.subject Surveillance en
dc.subject.lcsh Rickettsia en
dc.subject.lcsh Fleas en
dc.subject.lcsh Polymerase chain reaction en
dc.subject.lcsh Typhus, Endemic flea-borne en
dc.title Molecular detection of Rickettsia felis and Candidatus Rickettsia asemboensis in fleas from human habitats, Asembo, Kenya en
dc.type Postprint Article en

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