Viral replication kinetics and in vitro cytopathogenicity of parental and reassortant strains of bluetongue virus serotype 1, 6 and 8

Show simple item record Coetzee, Peter Van Vuuren, Moritz Stokstad, Maria Myrmel, Mette Van Gennip, Rene G.P. Van Rijn, Piet A. Venter, Estelle Hildegard 2014-05-23T09:18:32Z 2014-05-23T09:18:32Z 2014-06
dc.description.abstract Bluetongue virus (BTV), a segmented dsRNA virus, is the causative agent of bluetongue (BT), an economically important viral haemorrhagic disease of ruminants. Bluetongue virus can exchange its genome segments in mammalian or insect cells that have been co-infected with more than one strain of the virus. This process, may potentially give rise to the generation of novel reassortant strains that may differ from parental strains in regards to their phenotypic characteristics. To investigate the potential effects of reassortment on the virus’ phenotype, parental as well as reassortant strains of BTV serotype 1, 6, 8, that were derived from attenuated, and wild type strains by reverse genetics, were studied in vitro for their virus replication kinetics and cytopathogenicity in mammalian (Vero) cell cultures. The results indicate that genetic reassortment can affect viral replication kinetics, the cytopathogenicity and extent/mechanism of cell death in infected cell cultures. In particular, some reassortants of non-virulent vaccine (BTV-1 and BTV-6) and virulent field origin (BTV-8) demonstrate more pronounced cytopathic effects compared to their parental strains. Some reassortant strains in addition replicated to high titres in vitro despite being composed of genome segments from slow and fast replicating parental strains. The latter result may have implications for the level of viraemia in the mammalian host and subsequent uptake and transmission of reassortant strains (and their genome segments) by Culicoides vectors. Increased rates of CPE induction could further suggest a higher virulence for reassortant strains in vivo. Overall, these findings raise questions in regards to the use of modified-live virus (MLV) vaccines and risk of reassortment in the field. To further address these questions, additional experimental infection studies using insects and/or animal models should be conducted, to determine whether these results have significant implications in vivo. en
dc.description.librarian hb2014 en
dc.description.librarian ab2014
dc.description.sponsorship Norwegian State Educational Loan Fund (Lånekassen), Tine Dairy Cooperative (Norway), Animalia Meat and Poultry Research Centre (Norway), the IRT (Genomics) working group of the University of Pretoria, and the Ministry of Economic Affairs (CVI-project 1691022500), en
dc.description.uri en
dc.identifier.citation Coetzee, P, Van Vuuren, M, Stokstad, M, Myrmel, M, Van Gennip, RGP, Van Rijn, PA & Venter, EH 2014, 'Viral replication kinetics and in vitro cytopathogenicity of parental and reassortant strains of bluetongue virus serotype 1, 6 and 8 ', Veterinary Microbiology, vol. 171, no. 1-2, pp. 53-65. en
dc.identifier.issn 0378-1135 (print)
dc.identifier.issn 1873-2542 (online)
dc.identifier.other 10.1016/j.vetmic.2014.03.006
dc.identifier.other 7003904650
dc.identifier.other 7004572625
dc.identifier.other O-6953-2014
dc.identifier.other N-9080-2014
dc.language.iso en en
dc.publisher Elsevier en
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2014 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Veterinary Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Microbiology, vol. 171, no. 1-2, pp. 53-65, Jun 2014. doi : 10.1016/j.vetmic.2014.03.006. en
dc.subject Bluetongue en
dc.subject Reassortment en
dc.subject Cytopathogenicity en
dc.subject Replication kinetics en
dc.subject Apoptosis en
dc.subject Necrosis en
dc.subject Modified-live virus vaccine en
dc.subject BTV en
dc.subject Bluetongue en
dc.subject BT
dc.subject.lcsh Bluetongue virus en
dc.subject.lcsh Viruses -- Reproduction en
dc.title Viral replication kinetics and in vitro cytopathogenicity of parental and reassortant strains of bluetongue virus serotype 1, 6 and 8 en
dc.type Postprint Article en

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