Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity

Show simple item record Adewusi, Emmanuel Adekanmi Steenkamp, Paul Fouche, Gerda Steenkamp, Vanessa 2014-05-22T09:32:17Z 2014-05-22T09:32:17Z 2013-06
dc.description.abstract Boophone disticha (Amaryllidaceae) is widely used in traditional medicine in southern Africa. Several alkaloids, volatile oils and fatty acids have been isolated from the plant. However, there has been no literature report of a triterpene from B. disticha. Cycloeucalenol, a cycloartane triterpene, together with its regio-isomer, was isolated from the ethyl acetate extract of the bulbs using column chromatography and preparative thin layer chromatography. Structural elucidation was carried out using 1D and 2D NMR and mass spectroscopy. The MTT and neutral red assays were used to assess the cytotoxicity of the compound in human neuroblastoma (SH-SY5Y) cells. The compound was obtained as a mixture of two regio-isomers, which were separated for the first time by chromatographic optimization. Integration of the 1H NMR spectrum showed that cycloeucalenol and its regio-isomer were present in a ratio of 1.04:1. A dose-dependent decrease in cell viability was observed using both cytotoxicity assays. IC51 values of 173.0 +/- 5.1 microM and 223.0 +/- 6.4 microM were obtained for the MTT and neutral red assays, respectively, indicative of the low toxicity of the compound. This work describes for the first time, the presence of triterpene compounds from the genus Boophone. en_US
dc.description.librarian hb2014 en_US
dc.description.uri en_US
dc.identifier.citation Adewusi, EA., Steenkamp, P, Fouche, G & Steenkamp, V 2013, 'Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity', Natural Product Communications, vol. 8, no. 9, pp. 1213-1216. en_US
dc.identifier.issn 1934-578X (print)
dc.identifier.issn 1555-9475 (online)
dc.language.iso en en_US
dc.publisher Natural Product en_US
dc.rights Copyright 2013 Natural Product.This is a pre-print of an article accepted for publication in Natural Product Communications, vol. 8(9), pp 1213-1216. Natural Product Communcations is available at: en_US
dc.subject Amaryllidaceae en_US
dc.subject Boophone disticha en_US
dc.subject Cycloeucalenol en_US
dc.subject Cytotoxicity en_US
dc.subject SH-SY5Y cells en_US
dc.subject Regio-isomer en_US
dc.subject Traditional plants en_US
dc.title Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity en_US
dc.type Postprint Article en_US

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