Comparison of the new Mycofast Revolution assay with a molecular assay for the detection of genital mycoplasmas from clinical specimens 

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dc.contributor.author Redelinghuys, Mathys J.
dc.contributor.author Ehlers, M.M. (Marthie Magdaleen)
dc.contributor.author Dreyer, Andries W.
dc.contributor.author Lombaard, H.A.D.T. (Hennie)
dc.contributor.author Kock, Martha Magdalena
dc.date.accessioned 2014-04-11T12:16:43Z
dc.date.available 2014-04-11T12:16:43Z
dc.date.issued 2013-09-30
dc.description.abstract BACKGROUND: Genital mycoplasmas are opportunistic bacteria that are associated with undesirable gynaecologic and reproductive events. Mycoplasmas are fastidious bacteria with increasing resistance to routine antimicrobials and often fail to grow on conventional culture methods. The commercial Mycofast Revolution assay permits the phenotypic detection and identification of genital mycoplasmas. Antimicrobial susceptibility testing against five antimicrobial agents with MICs corresponding to the CLSI guidelines can also be performed. This study aimed to compare the new commercially available Mycofast Revolution assay with a multiplex PCR assay. METHODS: Self-collected swabs were obtained from pregnant women attending the antenatal clinic of a tertiary academic hospital in Pretoria, South Africa from October 2012 to November 2012. These swabs were used to seed UMMt and modified Amies transport media. The seeded UMMt transported medium was used to inoculate the Mycofast Revolution assay for the identification, enumeration and antimicrobial susceptibility testing of genital mycoplasmas. Following DNA extraction from the modified Amies transport medium, specimens were subjected to a multiplex PCR assay for the detection of genital mycoplasmas. RESULTS: The Mycofast Revolution kit had a sensitivity and specificity of 77.3% (95% CI: 62.15% to 88.51%) and 80% (95% CI: 28.81% to 96.70%), respectively, against the PCR assay. The positive and negative predictive values were 97.1% (95% CI: 85.03% to 99.52%) and 28.6% (95% CI: 8.57% to 58.08%). Genital mycoplasmas were detected in 71.4% (35/49) of samples with the Mycofast Revolution assay with 49% (24/49) being Ureaplasma spp. and 22.4% (11/49) mixed strains. The multiplex PCR assay had a positivity rate of 89.8% (44/49) for genital mycoplasmas; mixed strains were present in 51% (25/49) of samples, Ureaplasma spp. in 16.3% (8/49) and M. hominis in 22.4% (11/49) of samples. CONCLUSIONS: There was a fair agreement (κ = 0.319) between the Mycofast Revolution assay and the mPCR assay. With the high prevalence rates of genital mycoplasmas, fast and efficient diagnostic methods are imperative to treat infections and minimise complications. The Mycofast Revolution assay is simple to use, has a short turnaround time and interpretation of results are straightforward. This assay circumvents common problems experienced with conventional culture and molecular methods in diagnostic laboratories where skilled personnel are limited and can be used as an alternative diagnostic assay. en_US
dc.description.librarian am2014 en_US
dc.description.uri http://www.biomedcentral.com/1471-2334/13/453 en_US
dc.identifier.citation Redelinghuys et al.: Comparison of the new Mycofast Revolution assay with a molecular assay for the detection of genital mycoplasmas from clinical specimens. BMC Infectious Diseases 2013 13:453. en_US
dc.identifier.issn 1471-2334
dc.identifier.other 10.1186/1471-2334-13-453
dc.identifier.uri http://hdl.handle.net/2263/39653
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.rights © 2013 Redelinghuys et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_US
dc.subject Mycoplasma hominis en_US
dc.subject Ureaplasma spp en_US
dc.subject Mycofast en_US
dc.subject Antimicrobial susceptibilities en_US
dc.subject Multiplex polymerase chain reaction (M-PCR) en_US
dc.title Comparison of the new Mycofast Revolution assay with a molecular assay for the detection of genital mycoplasmas from clinical specimens  en_US
dc.type Article en_US


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