Inhaled microparticles containing clofazimine are efficacious in treatment of experimental tuberculosis in mice

Verma, Rahul Kumar; Germishuizen, Willem Andreas; Motheo, M.P. (Mamofalali Portia); Agrawal, Atul Kumar; Singh, Amit Kumar; Mohan, Mradul; Gupta, Pushpa; Gupta, Umesh Datta; Cholo, Moloko C.; Anderson, Ronald; Fouche, P. Bernard; Misra, Amit

Inhaled microparticles containing clofazimine are efficacious in treatment of experimental tuberculosis in mice

Verma, Rahul Kumar; Germishuizen, Willem Andreas; Motheo, M.P. (Mamofalali Portia); Agrawal, Atul Kumar; Singh, Amit Kumar; Mohan, Mradul; Gupta, Pushpa; Gupta, Umesh Datta; Cholo, Moloko C.; Anderson, Ronald; Fouche, P. Bernard; Misra, Amit

URI: http://hdl.handle.net/2263/39634

Date: 2013-02

Abstract:

Inhalable clofazimine-containing dry powder microparticles (CFM-DPI) and native clofazimine (CFM) were evaluated for activity against Mycobacterium tuberculosis in human monocyte-derived macrophage cultures and in mice infected with a low-dose aerosol. Both formulations resulted in 99% killing at 2.5 g/ml in vitro. In mice, 480 g and 720 g CFM-DPI inhaled twice per week over 4 weeks reduced numbers of CFU in the lung by as much as log10 2.6; 500 g oral CFM achieved a log10 0.7 reduction.

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