Colonization patterns of an mCherry-Tagged Pectobacterium carotovorum subsp. brasiliense Strain in potato plants

Show simple item record Kubheka, Gugulethu Cresentia Coutinho, Teresa A. Moleleki, Ntsane Moleleki, Lucy N. 2014-04-03T11:37:26Z 2014-04-03T11:37:26Z 2013
dc.description.abstract Pectobacterium carotovorum subsp. brasiliense is a newly identified member of the potato soft rot enterobacteriaceae. The pathogenesis of this pathogen is still poorly understood. In this study, an mCherry-P. carotovorum subsp. brasiliense-tagged strain was generated to study P. carotovorum subsp. brasiliense—potato plant interactions. Prior to use, the tagged strain was evaluated for in vitro growth, plasmid stability, and virulence on potato tubers and shown to be similar to the wild type. Four potato cultivars were evaluated for stem-based resistance against P. carotovorum subsp. brasiliense. Confocal laser-scanning microscopy and in vitro viable cell counts showed that P. carotovorum subsp. brasiliense is able to penetrate roots of a susceptible potato cultivar as early as 12 h postinoculation and migrate upward into aerial stem parts. Due to the phenotypic differences observed between tolerant and susceptible cultivars, a comparison of P. carotovorum subsp. brasiliense colonization patterns in these cultivars was undertaken. In the susceptible cultivar, P. carotovorum subsp. brasiliense cells colonized the xylem tissue, forming “biofilm-like” aggregates that led to occlusion of some of the vessels. In contrast, in the tolerant cultivar, P. carotovorum subsp. brasiliense appeared as free-swimming planktonic cells with no specific tissue localization. This suggests that there are resistance mechanisms in the tolerant cultivar that limit aggregation of P. carotovorum subsp. brasiliense in planta and, hence, the lack of symptom development in this cultivar. en_US
dc.description.librarian am2014 en_US
dc.description.sponsorship The National Research Foundation (Thuthuka Programme) of South Africa en_US
dc.description.uri en_US
dc.identifier.citation Kubheka, G. C., Coutinho, T. A., Moleleki, N., and Moleleki, L. N. 2013. Colonization patterns of an mCherry-tagged Pectobacterium carotovorum subsp. brasiliense strain in potato plants. Phytopathology 103:1268-1279. en_US
dc.identifier.issn 0031-9457 (print)
dc.identifier.issn 1851-5657 (online)
dc.identifier.other 10.1094/PHYTO-02-13-0049-R
dc.language.iso en en_US
dc.publisher Fundacion Romulo Raggio, Argentina en_US
dc.rights © 2013 The American Phytopathological Society en_US
dc.subject Pectobacterium carotovorum subsp. brasiliense en_US
dc.subject Colonization patterns en_US
dc.subject Potatoes en_US
dc.title Colonization patterns of an mCherry-Tagged Pectobacterium carotovorum subsp. brasiliense Strain in potato plants en_US
dc.type Article en_US

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