Expression of defence-related genes against Phytophthora cinnamomi in five avocado rootstocks

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dc.contributor.author Engelbrecht, Juanita
dc.contributor.author Van den Berg, Noelani
dc.date.accessioned 2014-04-03T11:34:12Z
dc.date.available 2014-04-03T11:34:12Z
dc.date.issued 2013-11
dc.description.abstract Avocado (Persea americana) – a major fruit crop worldwide – is threatened by root rot caused by Phytophthora cinnamomi. This pathogen is known to infect the plant via the feeder roots leading to branch dieback, and eventually tree mortality. While it is known that different avocado rootstocks have varying degrees of susceptibility to Phytophthora root rot, little research has been done on the avocado–Phytophthora interaction. In this study, transcript abundance levels of defence-related genes coding for phenylalanine ammonia-lyase, lipoxygenase, pathogenesis-related protein 5, endochitinase, gluthathionine S-transferase and metallothionein were characterised and compared among five rootstocks with varying susceptibility to root rot, after exposure to P. cinnamomi. Root samples were collected at 0 h, 3 h, 6 h, 12 h, 24 h, 48 h and 72 h post-infection and transcript abundance of the defence-related genes was determined using quantitative real-time reverse transcription PCR. The results indicated the involvement of PR-5 and endochitinase in the defence response of all avocado rootstocks to P. cinnamomi but these genes could not be directly linked to the observed phenotypic resistance. PR-5 and endochitinase were highly upregulated at 72 h post-infection. Differences in transcript abundance of phenylalanine ammonia-lyase and lipoxygenase genes were seen when comparing tolerant and less tolerant rootstocks, which may suggest that transcripts of these genes contribute to resistance. These data provide important insights into plant defence and into how different avocado rootstocks may exhibit increased resistance to infection by P. cinnamomi. en_US
dc.description.librarian am2014 en_US
dc.description.sponsorship The Hans Merensky Foundation and the THRIP programme of the National Research Foundation of South Africa. en_US
dc.description.uri http://www.sajs.co.za/ en_US
dc.identifier.citation Engelbrecht J, Van den Berg N. Expression of defencerelated genes against Phytophthora cinnamomi in five avocado rootstocks. S Afr J Sci. 2013;109(11/12), Art. #2012-0058, 8 pages. http://dx.DOI.org/ 10.1590/sajs.2013/20120058 en_US
dc.identifier.issn 0038-2353 (print)
dc.identifier.issn 1996-7489 (online)
dc.identifier.other 10.1590/sajs.2013/20120058
dc.identifier.uri http://hdl.handle.net/2263/39593
dc.language.iso en en_US
dc.publisher AOSIS Open Journals en_US
dc.rights © 2013. The Authors. Published under a Creative Commons Attribution Licence. en_US
dc.subject Phytophthora cinnamomi en_US
dc.subject Gene expression en_US
dc.subject Resistance en_US
dc.subject Avocado en_US
dc.subject Defence en_US
dc.title Expression of defence-related genes against Phytophthora cinnamomi in five avocado rootstocks en_US
dc.type Article en_US


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