Browsing South African National Veterinary Repository by Title

Browsing South African National Veterinary Repository by Title

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  • Isolation and preliminary characterization of a caprine rotavirus 
    Da Costa Mendes, Vasco Manuel; De Beer, M.C.; Goosen, G.H.; Steele, Andrew Duncan; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1994)
    Five cytopathic rotavirus strains were isolated in MA 104 cells from stool specimens of kids with diarrhoea. Pre-treatment of the virus with trypsin and incorporation of low levels of trypsin in the maintenance medium ...
  • Isolation and preliminary characterization of the jaagsiekte retrovirus (JSRV) 
    Verwoerd, Daniel Wynand; Payne, Anna-Lise; York, D.F.; Myer, M.S.; Bigalke, R.D. (Published by The Government Printer, Pretoria, 1983)
    Jaagsiekte, or ovine pulmonary adenomatosis, is caused by a recently discovered retrovirus. The virus cannot be cultivated in vitro at present, but a procedure is described for the isolation and purification of small amounts ...
  • The isolation and preliminary genetic classification of temperature-sensitive mutants of bluetongue virus 
    Shipham, S.O.; De la Rey, M.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Published by The Government Printer, Pretoria, 1976)
    Temperature-sensitive mutants of bluetongue virus were isolated and classified in 6 genetic recombination groups. The frequency of recombination varied both within and between groups. The 4 mutagens used viz. nitrous acid, ...
  • The isolation and serology of Brucella melitensis in a flock of goats in central RSA 
    Ribeiro, L.M.M.; Herr, S.; Chaparro, F.; Van der Vyver, F.H.; Verwoerd, Daniel Wynand (Published by the Government Printer, Pretoria., 1990)
    Brucella melitensis biotype 1 was isolated in pure culture from the lungs, liver, spleen, kidney, stomach contents, abomasum and brain of an aborted caprine (Boer goat) foetus in the district of Cullinan, near Pretoria. ...
  • The isolation and serology of the "FSA" Brucella melitensis Rev.1 mutant in a flock of sheep 
    Gummow, Bruce; Pefanis, S.M.; Pieterson, P.M.; Williamson, Catherine C.; Venter, Catharina G.; Herr, S.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1988)
    A flock of sheep, known to be infected with the "FSA" mutant of Brucella melitensis Rev. 1, was examined serologically and bacteriologically to determine whether any relationship existed which would help in the control of ...
  • Isolation and significance of anaerobic bacteria isolated from cases of bovine mastitis 
    Du Preez, J.H.; Greeff, A.S.; Eksteen, Nicolene; Steyn, P.J.J.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Published by The Government Printer, Pretoria, 1981)
    The role of obligate anaerobic bacteria in the aetiology of mastitis of lactating dairy cows was investigated. Anaerobes were isolated from 12% of lactating mastitic cows, which were representative of 50% of the 10 dairy ...
  • Isolation and transmission of an unidentified Babesia sp. infective for cattle 
    Thomas, Shan E.; Mason, T.E.; Steyn, P.J.J.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Published by The Government Printer, Pretoria, 1981)
    Engorged adult female ticks submitted from farms in South Africa were routinely screened for protozoan parasites by examination of haemolymph smears. An unidentified Babesia sp. was found in Hyalomma marginatum rufipes and ...
  • The isolation and transmission of an unidentified Babesia sp. to cattle by Hyalomma truncatum Koch 1844 
    De Waal, D.T.; Potgieter, F.T.; Combrink, M.P.; Mason, T.E.; Verwoerd, Daniel Wynand (Published by the Government Printer, Pretoria., 1990)
    An unidentified Babesia sp. which causes a mild disease in cattle was isolated in a splenectomized ox that received pooled blood from field cattle. That this organism is pleomorphic and resembles Babesia occultans makes ...
  • Isolation in mice and embryonated hen's eggs of a virus associated with vaginitis of cattle 
    McIntosh, B.M.; Haig, D.A.; Alexander, R.A.; Alexander, R.A.; Clark, R.; Louw, J.G.; De Kock, V.E. (Published by The Government Printer, Pretoria, 1954)
    From infected material obtained from various herds virus has been isolated in several instances in developing hen's eggs, and in one instance also in mice. The strain which could be propagated in both mice and eggs was ...
  • The isolation of a paramyxovirus from pigeons in South Africa 
    Pienaar, Anna C.E.; Cilliers, J.A.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    A paramyxovirus was isolated from diseased pigeons, submitted to the Poultry Section of the Veterinary Research Institute, Onderstepoort, for post-mortem examination. The mean death time for the isolates was determined ...
  • Isolation of Anaplasma marginale from Rhipicephalus simus males 
    Potgieter, F.T.; Van Rensburg, L.; Steyn, P.J.J.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1980)
    Approximately 100 adult Rhipicephalus simus from a batch known to be infected with Anaplasma marginale were used to infest an ox. Fifty male ticks were manually removed from the animal's ears 9 days after infestation. These ...
  • The isolation of Campylobacter hyointestinalis from a pig in South Africa 
    Van der Walt, Martha L.; Van der Lugt, Jaco J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1988)
    The isolation of a strain of Campylobacter hyointestinalis from a piglet is described. The animal originated from a farm where another animal showed signs of intestinal adenomatosis. The animal from which the Isolation was ...
  • The isolation of Cl. welchii, type B, from foals affected with dysentery 
    Mason, J.H.; Robinson, E.M.; Du Toit, P.J. (Pretoria : The Government Printer, 1938)
    An outbreak of dysentery in foals is recorded. The symptoms and post-mortem lesions have much in common with those of lamb dysentery. From the intestinal contents of two foals, Cl. welchii, type B (the lamb dysentery or ...
  • Isolation of Cowdria ruminantium by cellular affinity chromatography and detection by an enzyme-linked immunosorbent assay 
    Prozesky, Leon; Verschoor, J.A. (Jan Adrianus), 1953-; Neitz, Albert Walter Herman; Viljoen, Gerrit Johannes; Vermeulen, N.M.J.; Oberem, P.T.; Bezuidenhout, J. Dürr; Putterill, John Fraser; Visser, L.; Bigalke, R.D. (Published by The Government Printer, Pretoria, 1985)
    The isolation of Cowdria ruminantium by means of wheat germ lectin affinity chromatography as described in this paper permits the recovery of partially purified viable organisms under mild conditions in short time. These ...
  • Isolation of Cowdria ruminantium by means of Percoll density gradient centrifugation and detection by an enzyme-linked immunosorbent assay 
    Neitz, Albert Walter Herman; Verschoor, J.A. (Jan Adrianus), 1953-; Viljoen, G.J.; Bezuidenhout, J. Dürr; Oberem, P.T.; Putterill, John Fraser; Visser, L.; Vermeulen, N.M.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1986)
    The isolation of Cowdria ruminantium by means of Percoll density gradient centrifugation permits the recovery of partially purified viable populations of the organism possessing distinctly different densities. These ...
  • The isolation of Leptospira interrogans serovar pomona and related serological findings associated with a mixed farming unit in the Transvaal 
    Gummow, Bruce; De Lange, J.F.; Turner, G.V.; Redman, A.R.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    This is the first known isolation in the Republic of South Africa (RSA) of the serovar pomona from the organs of porcine foetuses as well as from the renal lymphnodes of slaughter pigs showing chronic nephritis. In addition, ...
  • The isolation of Mycoplasms synoviae from chickens with infectious synovitis and air-sacculitis in the Republic of South Africa 
    Buys, S.B.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Published by The Government Printer, Pretoria, 1976)
    Mycoplasma synoviae was isolated from the trachea of chickens showing either typical infectious synovitis lesions or air-sacculitis. M. synoviae was identified by means of the direct plate fluorescent antibody technique ...
  • The isolation of nucleic acid sequences specific for Cowdria ruminantium 
    Wilkins, S.C.; Ambrosio, R.E.; Verwoerd, Daniel Wynand (Published by the Government Printer, Pretoria., 1989)
    Screening of a genomic library of Cowdria ruminantium has yielded twelve clones hybridizing to Cowdria DNA. These clones should be suitable for development into DNA probes specific for this organism.
  • Isolation of pure Babesia equi and Babesia caballi organisms in splenectomized horses from endemic areas in South Africa 
    Stegmann, George F.; De Waal, D.T.; Van Heerden, J.; Van Den Berg, S.S.; Potgieter, F.T.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1988)
    Both Babesia equi and Babesia caballi are endemic in large parts of South Africa. Attempts were made to obtain pure local isolates of both B. equi and B. caballi for the purpose of developing serological tests to study the ...
  • Isolation of serovar C-3 Haemophilus paragallinarum from Zimbabwe: a further indication of the need for the production of vaccines against infectious coryza containing local isolates of H. paragallinarum 
    Bragg, R.R. (Robert Richard); Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Various isolates of Haemophilus paragallinarum, collected from a severe outbreak of infectious coryza in poultry from Zimbabwe, were serotyped and were found to belong to serovar C-3. Previously, isolates were serotyped ...