Fate of Salmonella Typhimurium in laboratory-scale drinking water biofilms

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dc.contributor.author Schaefer, L.M.
dc.contributor.author Brözel, V.S. (Volker Siegfried), 1963-
dc.contributor.author Venter, S.N. (Stephanus Nicolaas)
dc.date.accessioned 2014-04-01T08:13:14Z
dc.date.issued 2013-12
dc.description.abstract Investigations were carried out to evaluate and quantify colonization of laboratory-scale drinking water biofilms by a chromosomally green fluorescent protein (gfp)-tagged strain of Salmonella Typhimurium. Gfp encodes the green fluorescent protein and thus allows in situ detection of undisturbed cells and is ideally suited for monitoring Salmonella in biofilms. The fate and persistence of non-typhoidal Salmonella in simulated drinking water biofilms was investigated. The ability of Salmonella to form biofilms in monoculture and the fate and persistence of Salmonella in a mixed aquatic biofilm was examined. In monoculture S. Typhimurium formed loosely structured biofilms. Salmonella colonized established multi-species drinking water biofilms within 24 hours, forming micro-colonies within the biofilm. S. Typhimurium was also released at high levels from the drinking water-associated biofilm into the water passing through the system. This indicated that Salmonella could enter into, survive and grow within, and be released from a drinking water biofilm. The ability of Salmonella to survive and persist in a drinking water biofilm, and be released at high levels into the flow for recolonization elsewhere, indicates the potential for a persistent health risk to consumers once a network becomes contaminated with this bacterium. en_US
dc.description.librarian hb2014 en_US
dc.description.sponsorship This research was supported by a grant from the Water Research Commission of South Africa (WRC K5/1276) to VSB and SNV, and National Research Foundation (NRF) grant 2046811 to VSB. LMS was supported by a scholarship from the NRF of South Africa. en_US
dc.description.uri http://www.iwaponline.com/jwh/default.htm en_US
dc.identifier.citation Schaefer, LM, Brozel, VS & Venter, SN 2013, 'Fate of Salmonella Typhimurium in laboratory-scale', Journal of Water and Health, vol. 11, no. 4, pp. 629-635. en_US
dc.identifier.issn 1477-8920 (print)
dc.identifier.other 10.2166/wh.2013.208
dc.identifier.uri http://hdl.handle.net/2263/37247
dc.language.iso en en_US
dc.publisher IWA Publishing en_US
dc.rights © IWA Publishing 2013. The definitive peer-reviewed and edited version of this article is published in Journal of Water and Health, vol.11, no. 4, pp. 629-635, 2013. doi : 10.2166/wh.2013.208 and is available at : http://www.iwaponline.com/jwh/default.htm en_US
dc.subject Salmonella Typhimurium en_US
dc.subject Laboratory scale en_US
dc.subject Drinking water en_US
dc.title Fate of Salmonella Typhimurium in laboratory-scale drinking water biofilms en_US
dc.type Postprint Article en_US


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