Global distribution of Bartonella infections in domestic bovine and characterization of Bartonella bovis strains using multi-locus sequence typing

Show simple item record Bai, Ying Malania, Lile Castillo, Danilo Alvarez Moran, David Boonmar, Sumalee Chanlun, Aran Suksawat, Fanan Maruyama, Soichi Knobel, Darryn Leslie Kosoy, Michael 2014-02-26T08:02:11Z 2014-02-26T08:02:11Z 2013-11-21
dc.description.abstract Bartonella bovis is commonly detected in cattle. One B. bovis strain was recently isolated from a cow with endocarditis in the USA, suggesting its role as an animal pathogen. In the present study, we investigated bartonella infections in 893 cattle from five countries (Kenya, Thailand, Japan, Georgia, and Guatemala) and 103 water buffaloes from Thailand to compare the prevalence of the infection among different regions and different bovid hosts. We developed a multi-locus sequence typing (MLST) scheme based on nine loci (16S rRNA, gltA, ftsZ, groEL, nuoG, ribC, rpoB, ssrA, and ITS) to compare genetic divergence of B. bovis strains, including 26 representatives from the present study and two previously described reference strains (one from French cows and another from a cow with endocarditis in the USA). Bartonella bacteria were cultured in 6.8% (7/103) of water buffaloes from Thailand; all were B. bovis. The prevalence of bartonella infections in cattle varied tremendously across the investigated regions. In Japan, Kenya, and the Mestia district of Georgia, cattle were free from the infection; in Thailand, Guatemala, and the Dusheti and Marneuli districts of Georgia, cattle were infected with prevalences of 10–90%. The Bartonella isolates from cattle belonged to three species: B. bovis (n=165), B. chomelii (n=9), and B. schoenbuchensis (n=1), with the latter two species found in Georgia only. MLST analysis suggested genetic variations among the 28 analyzed B. bovis strains, which fall into 3 lineages (I, II, and III). Lineages I and II were found in cattle while lineage III was restricted to water buffaloes. The majority of strains (17/28), together with the strain causing endocarditis in a cow in the USA, belonged to lineage I. Further investigations are needed to determine whether B. bovis causes disease in bovids. en
dc.description.librarian am2014 en
dc.description.librarian ab2014 en
dc.description.sponsorship Specimens from cattle in Kenya were collected under a project supported by the Wellcome Trust, UK (grant number 081828/B/06/Z). en
dc.description.uri en
dc.identifier.citation Bai Y, Malania L, Alvarez Castillo D, Moran D, Boonmar S, et al. (2013) Global Distribution of Bartonella Infections in Domestic Bovine and Characterization of Bartonella bovis Strains Using Multi-Locus Sequence Typing. PLoS ONE 8(11): e80894. DOI: 10.1371/journal.pone.0080894 en
dc.identifier.issn 1932-6203
dc.identifier.other 10.1371/journal.pone.0080894
dc.identifier.other 6602518021
dc.identifier.other O-7057-2014
dc.identifier.other 0000-0002-0425-3799
dc.language.iso en en
dc.publisher Public Library of Science en
dc.relation.requires Adobe Acrobat Reader en
dc.rights The work is made available under the Creative Commons CC0 public domain dedication. en
dc.subject Bartonella bovis en
dc.subject Global distribution en
dc.subject Investigations en
dc.subject.lcsh Cattle -- Diseases en
dc.subject.lcsh Bartonella infections in animals en
dc.subject.lcsh Bacterial diseases in animals en
dc.title Global distribution of Bartonella infections in domestic bovine and characterization of Bartonella bovis strains using multi-locus sequence typing en
dc.type Article en

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