Protein profiles of phosphorus- and nitrate-removing activated sludge systems

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Authors

Ehlers, Marthie Magdaleen
Cloete, Thomas Eugene

Journal Title

Journal ISSN

Volume Title

Publisher

Water Research Council

Abstract

Samples from laboratory-scale activated sludge systems operating under spesific conditions which differ in phosphate-removing capabilities were obtained from the University of Cape Town. The total protein content of samples of these systems was used and the protein separated with SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Analysis of the total proteins was employed as a fingerprint to type and compare the diversity of the bacterial communities of P-and N-removing systems. Samples of six activated slugde systems (three N-and P-removing and three N-removing) were used in this study. Protein profiles indicated a high (>70%) correlation for all the systems. No difference was observed in the protein profiles of the bacterial communities of N-and P-removing or N-removing systems. We can therefore conclude that the same bacterial communities were present in P-and N-removing systems.

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Keywords

Sludge systems, Protein profiles, Phosphorus- and nitrate-removing

Sustainable Development Goals

Citation

Ehlers, MM & Cloete, TE 1999, Protein profiles of phosphorus- and nitrate-removing activated sludge systems, Water SA, vol. 25, no. 3, pp. 351-356. [http://www.wrc.org.za/publications_watersa.htm]